Invitrogen

STIM1 Monoclonal Antibody (CDN3H4)

STIM1 Antibody in Immunocytochemistry (ICC/IF)

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STIM1 Antibody (MA1-19451) in ICC/IF

Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red) Monoclonal antibody (Product # MA1-19451). Nuclei were stained with DAPI (blue).

STIM1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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产品信息

MA1-19451

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CDN3H4

抗原

Synthetic peptide corresponding to the C-terminal cytoplasmic part of STIM1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2197884

产品详细信息

This antibody reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Western Blot: Both reducing and non-reducing conditions; Immunocytochemistry: Methanol-acetone fixation.

靶标信息

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Proteomic Analyses of the G Protein-Coupled Estrogen Receptor GPER1 Reveal Constitutive Links to Endoplasmic Reticulum, Glycosylation, Trafficking, and Calcium Signaling.

Cells

Ahmadian Elmi M,Motamed N,Picard D

MA1-19451 was used in Western Blot to show that the Ca2+ sensor STIM1 interacts with GPER1 and that upon STIM1 overexpression and depletion of Ca2+ stores, GPER1 becomes more nuclear.

Fri Nov 03 00:00:00 UTC 2023

Proteomic Analyses of the G Protein-Coupled Estrogen Receptor GPER1 Reveal Constitutive Links to Endoplasmic Reticulum, Glycosylation, Trafficking, and Calcium Signaling.

Cells

Ahmadian Elmi M,Motamed N,Picard D

MA1-19451 was used in Western Blot to show that the Ca2+ sensor STIM1 interacts with GPER1 and that upon STIM1 overexpression and depletion of Ca2+ stores, GPER1 becomes more nuclear.

Fri Nov 03 00:00:00 UTC 2023

Immune Synapse Residency of Orai1 Alters Ca²⁺ Response of T Cells.

International journal of molecular sciences

Voros O,Panyi G,Hajdu P

Published figure using STIM1 monoclonal antibody (Product # MA1-19451) in Western Blot

Tue Oct 26 00:00:00 UTC 2021

Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.

eLife

Tammineni ER,Kraeva N,Figueroa L,Manno C,Ibarra CA,Klip A,Riazi S,Rios E

MA1-19451 was used in Immunohistochemistry to reveal that Malignant Hyperthermia Susceptibility entails a wide-ranging, pathogenic change in glucose metabolism.

Mon May 04 00:00:00 UTC 2020

Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.

eLife

Tammineni ER,Kraeva N,Figueroa L,Manno C,Ibarra CA,Klip A,Riazi S,Rios E

MA1-19451 was used in Immunohistochemistry to reveal that Malignant Hyperthermia Susceptibility entails a wide-ranging, pathogenic change in glucose metabolism.

Mon May 04 00:00:00 UTC 2020

STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.

The Journal of cell biology

Sun J,Lu F,He H,Shen J,Messina J,Mathew R,Wang D,Sarnaik AA,Chang WC,Kim M,Cheng H,Yang S

MA1-19451 was used in immunohistochemistry - paraffin section to elucidate how STIM1- and Orai1-mediated calcium oscillations promote melanoma invasion

Mon Nov 24 00:00:00 UTC 2014

STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.

The Journal of cell biology

Sun J,Lu F,He H,Shen J,Messina J,Mathew R,Wang D,Sarnaik AA,Chang WC,Kim M,Cheng H,Yang S

MA1-19451 was used in immunohistochemistry - paraffin section to elucidate how STIM1- and Orai1-mediated calcium oscillations promote melanoma invasion