Invitrogen

STRAP Polyclonal Antibody

STRAP Antibody in Immunocytochemistry (ICC/IF)

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STRAP Antibody (PA5-119874) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of Unrip in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with STRAP Polyclonal Antibody (Product # PA5-119874) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

STRAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-119874

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:50-1:100

流式细胞分析 (Flow)

1:500-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within C-terminal human Unrip from aa 200-350

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913446

产品详细信息

Positive Control: HepG2 cell lysate, Hela cell lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, A431 cell lysate, rat brain tissue lysate, human placenta tissue, mouse colon tissue, SH-SY5Y, Hela.

Subcellular Location: Nucleus, Cytoplasm.

靶标信息

STRAP is a 350 amino acid MAP activator with WD-40 repeats that is localized both in the cytoplasm and the nucleus. It is a ubiquitously expressed protein that acts as an inhibitor of TGF-beta signaling and an important regulator of cell proliferation. Stable expression of STRAP results in activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and in down-regulation of the cyclin-dependent kinase inhibitor p21 (Cip1), which results in retinoblastoma protein hyperphosphorylation. Upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. It is the first component of the U snRNP assembly machinery that associates with SMN complex in a compartment-specific way and plays a crucial role in the intracellular distribution of SMN1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。