Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
14-6688-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Rat
已发表种属
Dog,
Human,
Rat
宿主/亚型
Mouse
/ IgM, lambda
分类
Monoclonal
类型
Antibody
克隆号
STRO-1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572902
产品详细信息
Description: The monoclonal antibody STRO-1 recognizes STRO-1, a bone marrow (immature) mesenchymal cell surface protein. Pluripotent stromal cells can give rise to several lineages: osteoblasts and chondrocytes, which form bone, adipocytes or hematopoietic cells. STRO-1+ cells when co-transplanted with hematopoietic stem cells have been shown to reduce the occurrence of graft versus host disease. In addition, in vitro cultures of Stro-1+ cells along with CD34+ cells give rise to CFUs (colony forming units) composed of hematopoietic precursors, establishing a supporting role of these mesenchymal cells in blood cell development.
The STRO-1 antibody has been reported to recognize both bovine and rat Stro-1 protein.
Applications Reported: This STRO-1 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This STRO-1 antibody has been tested by immunocytochemistry on methanol-fixed MG-63 cells and can be used at less than or equal to 20 µg/mL. The STRO-1 antibody has also been tested by flow cytometry on MG-63 cells and can be used at less than or equal to 0.5 µg/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: STRO1
