Invitrogen

STUB1 Polyclonal Antibody

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STUB1 Antibody (PA1-015) in WB

Western blot analysis of CHIP was performed by loading 40 µg of MCF-7 cell lysates and 3 µL PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 10% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk/PBS-T for 1 h at room temperature. CHIP was detected at 35 kDa using a CHIP antibody (Product # PA1-015) at 1:1000 dilution in 5% BSA/PBS-T buffer overnight at 4°C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution in 5% skim milk/PB... View More

STUB1 Antibody in Immunocytochemistry (ICC/IF)

STUB1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

STUB1 Antibody — FIG. 1. Knockdown of endogenous CHIP increases iNOS cellular level. (A). HEK293 cells stably expressing iNOS were transfected for 72 h with a plasmid encoding either control shRNA having no perfect matches to any human gene or shRNA specific for CHIP. (B) RT4 cells were transduced with lentivirus encoding CHIP-specific shRNA or control shRNA. After 48 h, cells were incubated with gamma interferon (100 U/ml), interleukin-1beta (0.5 ng/ml), and tumor necrosis factor alpha (10 ng/ml) for 24 h. In both panels, cells were lysed, and Western analysis was used to evaluate CHIP, iNOS, or GAPDH. iNOS activity was evaluated by measuring nitrite accumulation in the culture medium. Data represent mean +- standard deviation. n = 3 (A) or 4 (B). *, P < 0.05, compared to the control condition. {KD}

Product Details

PA1-015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2271290

Product Specific Information

PA1-015 detects human and mouse carboxyl terminus of hsc70-interacting protein (CHIP).

PA1-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~35 kDa protein representing CHIP from COS-1 cells overexpressing the human gene and a non-specific band at ~85 kDa.

PA1-015 was used successfully in the western blot analysis of CHIP using human MCF7 cell lysates.

The PA1-015 immunogen is a synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP. This peptide (Cat. # PEP-179) is available for use in neutralization and control experiments.

Target Information

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient Cancer.

Cancer cell

McGrail DJ,Garnett J,Yin J,Dai H,Shih DJH,Lam TNA,Li Y,Sun C,Li Y,Schmandt R,Wu JY,Hu L,Liang Y,Peng G,Jonasch E,Menter D,Yates MS,Kopetz S,Lu KH,Broaddus R,Mills GB,Sahni N,Lin SY

PA1-015 was used in Immunohistochemistry to demonstrate if deficient DNA mismatch repair induces destabilizing mutations leading to proteome instability in deficient DNA mismatch repair tumors.

Mon Mar 16 00:00:00 UTC 2020

S-Nitrosylation of CHIP Enhances F508Del-CFTR Maturation.

American journal of respiratory cell and molecular biology

Zaman K,Knight J,Hussain F,Cao R,Estabrooks SK,Altawallbeh G,Holloway K,Jafri A,Sawczak V,Li Y,Getsy P,Sun F,Raffay T,Cotton C,Brodsky JL,Periasamy A,Lewis SJ,Gaston B

PA1-015 was used in Western Blot, Immunoprecipitation to examine the interaction between S-nitrosothiols(SNOs) and the molecular cochaperone C-terminus Hsc70 interacting protein (CHIP), which is an E3 ubiquitin ligase that targets improperly folded CF transmembrane conductance regulator (CFTR) for subsequent degradation.

Sun Dec 01 00:00:00 UTC 2019

S-Nitrosylation of CHIP Enhances F508Del-CFTR Maturation.

American journal of respiratory cell and molecular biology

Zaman K,Knight J,Hussain F,Cao R,Estabrooks SK,Altawallbeh G,Holloway K,Jafri A,Sawczak V,Li Y,Getsy P,Sun F,Raffay T,Cotton C,Brodsky JL,Periasamy A,Lewis SJ,Gaston B

Sun Dec 01 00:00:00 UTC 2019

On the Mechanism of Hyperthermia-Induced BRCA2 Protein Degradation.

Cancers

van den Tempel N,Zelensky AN,Odijk H,Laffeber C,Schmidt CK,Brandsma I,Demmers J,Krawczyk PM,Kanaar R

Published figure using STUB1 polyclonal antibody (Product # PA1-015) in Western Blot

Tue Jan 15 00:00:00 UTC 2019

Modulation of heme/substrate binding cleft of neuronal nitric-oxide synthase (nNOS) regulates binding of Hsp90 and Hsp70 proteins and nNOS ubiquitination.

The Journal of biological chemistry

Peng HM,Morishima Y,Pratt WB,Osawa Y

PA1-015 was used in western blot to investigate the mechanism for neuronal nitric-oxide synthase activity and degradation

Fri Jan 06 00:00:00 UTC 2012

Brain distribution of carboxy terminus of Hsc70-interacting protein (CHIP) and its nuclear translocation in cultured cortical neurons following heat stress or oxygen-glucose deprivation.

Cell stress & chaperones

Anderson LG,Meeker RB,Poulton WE,Huang DY

Published figure using STUB1 polyclonal antibody (Product # PA1-015) in Immunofluorescence

Wed Sep 01 00:00:00 UTC 2010

Inhibition of hsp70 by methylene blue affects signaling protein function and ubiquitination and modulates polyglutamine protein degradation.

The Journal of biological chemistry

Wang AM,Morishima Y,Clapp KM,Peng HM,Pratt WB,Gestwicki JE,Osawa Y,Lieberman AP

PA1-015 was used in blocking/activating experiment to investigate the effect of methylene blue-induced HSP70 inhibition on protein functions and degradation

Fri May 21 00:00:00 UTC 2010

A critical role for CHIP in the aggresome pathway.

Molecular and cellular biology

Sha Y,Pandit L,Zeng S,Eissa NT

Published figure using STUB1 polyclonal antibody (Product # PA1-015) in Western Blot

Thu Jan 01 00:00:00 UTC 2009

A critical role for CHIP in the aggresome pathway.

Molecular and cellular biology

Sha Y,Pandit L,Zeng S,Eissa NT

Published figure using STUB1 polyclonal antibody (Product # PA1-015) in Western Blot

Thu Jan 01 00:00:00 UTC 2009

Regulation of death-associated protein kinase. Stabilization by HSP90 heterocomplexes.

The Journal of biological chemistry

Zhang L,Nephew KP,Gallagher PJ

PA1-015 was used in western blot to study the role of death-associated protein kinase DAPK in apoptosis and cellular homeostasis.

Fri Apr 20 00:00:00 UTC 2007

CHIP (carboxyl terminus of Hsc70-interacting protein) promotes basal and geldanamycin-induced degradation of estrogen receptor-alpha.

Molecular endocrinology (Baltimore, Md.)

Fan M,Park A,Nephew KP

PA1-015 was used in western blot to demonstrate the effect of carboxyl terminus of Hsc70-interacting protein on basal and geldanamycin-induced degradation of estrogen receptor-alpha.

Thu Dec 01 00:00:00 UTC 2005

CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival.

The Journal of biological chemistry

Shimura H,Schwartz D,Gygi SP,Kosik KS

PA1-015 was used in western blot to investigate the role of CHIP-Hsc70 complex in regulating phosphorylated tau-induced cell death

Fri Feb 06 00:00:00 UTC 2004

Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions.

Molecular and cellular biology

Ballinger CA,Connell P,Wu Y,Hu Z,Thompson LJ,Yin LY,Patterson C

PA1-015 was used in immunoprecipitation to investigate the interaction between CHIP and Hsc70 as well as Hsp70

Tue Jun 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); LA16c-313D11.6; RING-type E3 ubiquitin transferase CHIP; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase

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Gene Aliases: 0610033N24Rik; 2210017D18Rik; 2310040B03Rik; AW046544; CHIP; HSPABP2; NY-CO-7; PP1131; SCAR16; SDCCAG7; STUB1; UBOX1

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UniProt ID: (Human) Q9UNE7, (Mouse) Q9WUD1

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Entrez Gene ID: (Human) 10273, (Mouse) 56424

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Function(s)

G-protein coupled receptor binding ubiquitin-protein transferase activity protein binding ligase activity enzyme binding kinase binding Hsp70 protein binding protein binding, bridging TPR domain binding ubiquitin protein ligase binding ubiquitin-ubiquitin ligase activity protein homodimerization activity SMAD binding misfolded protein binding Hsp90 protein binding ubiquitin protein ligase activity ubiquitin protein ligase activity involved in ERAD pathway UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity heat shock protein binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination DNA repair ubiquitin-dependent protein catabolic process misfolded or incompletely synthesized protein catabolic process protein ubiquitination negative regulation of transforming growth factor beta receptor signaling pathway ubiquitin-dependent SMAD protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination regulation of protein stability regulation of glucocorticoid metabolic process negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process ERAD pathway ERBB2 signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-protein transferase activity protein maturation protein autoubiquitination protein K63-linked ubiquitination cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly protein folding cellular response to DNA damage stimulus

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STUB1 Polyclonal Antibody

STUB1 Antibody (PA1-015) in WB

Product Details

PA1-015

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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