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SUCLA2 Antibody (A305-477A) in WB
Detection of human and mouse SUCLA2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUCLA2 antibody (Product # A305-477A) (lot A305-477A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-477A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 to 100 of Human Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Dog and Pig
Target Information
ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-SCS; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta; renal carcinoma antigen NY-REN-39; RP11-528D24.2; SCS-betaA; SCSbetaA; succinate--CoA ligase (ADP-forming); Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial; succinate-CoA ligase beta subunit; succinate-CoA ligase, ADP-forming, beta subunit; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; Succinyl-CoA synthetase beta-A chain
Gene Aliases: 4930547K18Rik; A-BETA; MTDPS5; SCS-betaA; SUCLA2
UniProt ID: (Human) Q9P2R7, (Mouse) Q9Z2I9
Entrez Gene ID: (Human) 8803, (Mouse) 20916
succinate-CoA ligase (ADP-forming) activity
protein binding
ATP binding
metal ion binding
nucleotide binding
catalytic activity
acyl-CoA ligase activity
succinate-CoA ligase activity
3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid CoA ligase activity
fatty acid ligase activity
ligase activity
3-isopropenyl-6-oxoheptanoyl-CoA synthetase activity
2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA synthetase activity
benzoyl acetate-CoA ligase activity
2,4-dichlorobenzoate-CoA ligase activity
pivalate-CoA ligase activity
cyclopropanecarboxylate-CoA ligase activity
adipate-CoA ligase activity
citronellyl-CoA ligase activity
mentha-1,3-dione-CoA ligase activity
thiophene-2-carboxylate-CoA ligase activity
2,4,4-trimethylpentanoate-CoA ligase activity
cis-2-methyl-5-isopropylhexa-2,5-dienoate-CoA ligase activity
trans-2-methyl-5-isopropylhexa-2,5-dienoate-CoA ligase activity
branched-chain acyl-CoA synthetase (ADP-forming) activity
aryl-CoA synthetase (ADP-forming) activity
3-hydroxypropionyl-CoA synthetase activity
perillic acid:CoA ligase (ADP-forming) activity
perillic acid:CoA ligase (AMP-forming) activity
(3R)-3-isopropenyl-6-oxoheptanoate:CoA ligase (ADP-forming) activity
(3R)-3-isopropenyl-6-oxoheptanoate:CoA ligase (AMP-forming) activity
pristanate-CoA ligase activity
malonyl-CoA synthetase activity
ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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