Invitrogen

SWAP70 Polyclonal Antibody

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SWAP70 Antibody (PA5-30559) in WB

Western Blot analysis of SWAP70 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SWAP70 Polyclonal Antibody (Product # PA5-30559) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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产品信息

PA5-30559

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 220 of Human SWAP70

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.7 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2548033

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Raji.

Predicted reactivity: Mouse (95%), Rat (95%), Chicken (85%), Rhesus Monkey (99%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SWAP70 is a 585 amino acid regulatory protein belonging to the pleckstrin-like family with a PH domain essential for phosphatidylinositol 3, 4, 5-trisphosphate binding. SWAP70 acts as a link between the recognition of specific switch regions and causation of a DNA break and is involved in both nuclear events and in signaling B-cell activation. As a guanine nucleotide exchange factor (GEF) it transduces signals from tyrosine kinase receptors to RAC, independent of RAS. As a multiple functional signaling protein, and a component of SWAP enzyme complex, it mediates signaling of membrane ruffling by tyrosine kinase growth factor receptors, actin cytoskeleton and cell protrusion. It is widely expressed in most tissues, mature B-cells, including those associated with mucosa-associated tissue and bronchus-associated tissue, monocytes and macrophages.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。