Invitrogen

SYT11 Polyclonal Antibody

1个已发表图片

4 篇参考文献

查看其他5个SYT11抗体

抗体检测数据 (4)
已发表图片 (1)

SYT11 Antibody in Immunocytochemistry (ICC/IF)

SYT11 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

SYT11 Antibody (PA5-96970) in ICC/IF

Immunofluorescence analysis of SYT11 in mouse brain cells. Samples were incubated with SYT11 Polyclonal antibody (Product # PA5-96970) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

SYT11 Antibody in Immunocytochemistry (ICC/IF)

SYT11 Antibody in Immunocytochemistry (ICC/IF)

SYT11 Antibody in Immunocytochemistry (ICC/IF)

SYT11 Antibody in Western Blot (WB)

SYT11 Antibody in Western Blot (WB)

Product Image Gallery

SYT11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SYT11 in mouse brain cells. Samples were incubated with SYT11 Polyclonal antibody (Product # PA5-96970) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

SYT11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SYT11 in rat brain cells. Samples were incubated with SYT11 Polyclonal antibody (Product # PA5-96970) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

SYT11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SYT11 in SH-SY5Y cells. Samples were incubated with SYT11 Polyclonal antibody (Product # PA5-96970) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

SYT11 Antibody in Western Blot (WB) — Western blot analysis of SYT11 in extracts of various cell lines. Samples were incubated with SYT11 Polyclonal antibody (Product # PA5-96970) using a dilution of 1:600, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

SYT11 Antibody in Western Blot (WB) — Figure 5. Impad1 interacts with another trafficking protein, Syt11 (A) Venn diagram depicting the Impad1 inter-actome identified by IP-MS analysis. See also Table S2 . (B) Probing for Syt11 upon immunoprecipitation(IP) of FLAG-Impad1 in 393P vector and Impad1overexpressing cells. Immunoglobulin G (IgG) IP was used as a negative control. (C) Probing for Syt11 and Impad1 upon IP of Syt11 in 393P vector and Impad1-overexpressing cells. (D) Probing for Syt11 and Impad1 upon Syt11 IP in 344SQ scramble and shSyt11 cells. (E) Western blot analysis of Syt11 in human NSCLC cell lines distinguished by EMT status. (F) Correlation of Syt11 with EMT score (Spearman rho, 0.529; p = 1.16e07) in human NSCLC cell lines. See also Figure S5 .

产品信息

PA5-96970

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_6894933)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.67 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808772

产品详细信息

Immunogen sequence: WSCCHQQAEK KQKNPPYKFI HMLKGISIYP ETLSNKKKII KVRRDKDGPG REGGRRNLLV DAAEAGLLSR DKDPRGPSSG SCIDQLPIKM DYGEELRSPI TSLTPGESKT TSPSSPEEDV MLGSLTFSVD YNFPKKALVV TIQEAHGLPV MDDQTQGSDP YIKM; Positive Samples: U-251MG, Mouse brain, Rat brain; Cellular Location: Cell junction, Cytoplasmic vesicle, Membrane, Single-pass membrane protein, secretory vesicle, synapse, synaptic vesicle membrane

靶标信息

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature. [provided by RefSeq, Apr 2010].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Cell reports

Bajaj R,Rodriguez BL,Russell WK,Warner AN,Diao L,Wang J,Raso MG,Lu W,Khan K,Solis LS,Batra H,Tang X,Fradette JF,Kundu ST,Gibbons DL

PA5-96970 was used in Immunohistochemistry and Western Blotting to identify Impad1 as a regulator of EMT and secretome-mediated changes during lung cancer progression.

Tue Sep 27 00:00:00 UTC 2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Cell reports

Bajaj R,Rodriguez BL,Russell WK,Warner AN,Diao L,Wang J,Raso MG,Lu W,Khan K,Solis LS,Batra H,Tang X,Fradette JF,Kundu ST,Gibbons DL

PA5-96970 was used in Immunohistochemistry and Western Blotting to identify Impad1 as a regulator of EMT and secretome-mediated changes during lung cancer progression.

Tue Sep 27 00:00:00 UTC 2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Cell reports

Bajaj R,Rodriguez BL,Russell WK,Warner AN,Diao L,Wang J,Raso MG,Lu W,Khan K,Solis LS,Batra H,Tang X,Fradette JF,Kundu ST,Gibbons DL

PA5-96970 was used in Immunohistochemistry and Western Blotting to identify Impad1 as a regulator of EMT and secretome-mediated changes during lung cancer progression.

Tue Sep 27 00:00:00 UTC 2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Cell reports

Bajaj R,Rodriguez BL,Russell WK,Warner AN,Diao L,Wang J,Raso MG,Lu W,Khan K,Solis LS,Batra H,Tang X,Fradette JF,Kundu ST,Gibbons DL

PA5-96970 was used in Immunohistochemistry and Western Blotting to identify Impad1 as a regulator of EMT and secretome-mediated changes during lung cancer progression.

Tue Sep 27 00:00:00 UTC 2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Cell reports

Bajaj R,Rodriguez BL,Russell WK,Warner AN,Diao L,Wang J,Raso MG,Lu W,Khan K,Solis LS,Batra H,Tang X,Fradette JF,Kundu ST,Gibbons DL

PA5-96970 was used in Immunohistochemistry and Western Blotting to identify Impad1 as a regulator of EMT and secretome-mediated changes during lung cancer progression.

Tue Sep 27 00:00:00 UTC 2022

Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.

Protein & cell

Liang D,Xue Z,Xue J,Xie D,Xiong K,Zhou H,Zhang F,Su X,Wang G,Zou Q,Liu Y,Yang J,Ma H,Peng L,Zeng C,Li G,Wang L,Chen YH

PA5-96970 was used in Immunohistochemistry (Paraffin), Immunocytochemistry to suggest that SANPCs share dominant biological properties with glutamatergic neurons, and the glutamatergic neurotransmitter system may act as an intrinsic regulation module of heart rhythm, which provides a potential intervention target for pacemaker cell-associated arrhythmias.

Thu Jul 01 00:00:00 UTC 2021

Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.

Protein & cell

Liang D,Xue Z,Xue J,Xie D,Xiong K,Zhou H,Zhang F,Su X,Wang G,Zou Q,Liu Y,Yang J,Ma H,Peng L,Zeng C,Li G,Wang L,Chen YH

PA5-96970 was used in Immunohistochemistry (Paraffin), Immunocytochemistry to suggest that SANPCs share dominant biological properties with glutamatergic neurons, and the glutamatergic neurotransmitter system may act as an intrinsic regulation module of heart rhythm, which provides a potential intervention target for pacemaker cell-associated arrhythmias.

Thu Jul 01 00:00:00 UTC 2021

生物信息学

蛋白别名: synaptotagmin 11; synaptotagmin 12; Synaptotagmin XI; Synaptotagmin-11; SytXI

查看更多收起

基因别名: 1500004A13Rik; 3632445O20Rik; 5430404N14Rik; 6530420C11Rik; AI851970; KIAA0080; SYT11; SYT12; sytXI

查看更多收起

UniProt ID: (Human) Q9BT88, (Mouse) Q9R0N3, (Rat) O08835

查看更多收起

Entrez Gene ID: (Human) 23208, (Mouse) 229521, (Rat) 60568

查看更多收起

calcium ion binding protein binding calcium-dependent phospholipid binding syntaxin binding clathrin binding ubiquitin protein ligase binding protein homodimerization activity metal ion binding transporter activity membrane trafficking regulatory protein

plasma membrane repair vesicle fusion regulation of calcium ion-dependent exocytosis negative regulation of neurotransmitter secretion calcium ion-regulated exocytosis of neurotransmitter protein homotetramerization negative regulation of interleukin-6 secretion negative regulation of tumor necrosis factor secretion regulation of phagosome maturation positive regulation of protein localization to phagocytic vesicle calcium ion regulated lysosome exocytosis synaptic vesicle exocytosis transport