Invitrogen

Secretin Polyclonal Antibody

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Secretin Antibody (PA5-35973) in WB

Western Blot analysis of Secretin was performed by separating 30 µg of non-transfected (–) and transfected (+) unboiled 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Secretin Polyclonal Antibody (Product # PA5-35973) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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产品信息

PA5-35973

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 28 and 54 of Secretin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

AB_2553283

产品详细信息

Recommended positive controls: unboiled DDDDK-tagged SCT-transfected 293T cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。