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Separase Antibody (PA5-120352) in WB
Western blot analysis of Separase in extracts of various cell lines. Samples were incubated with Separase Polyclonal antibody (Product # PA5-120352) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-120352
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4).
偶联物
形式
Liquid
浓度
1.34 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2913924
产品详细信息
Positive test controls include: HeLa, Jurkat, Mouse spleen, Rat spleen, Rat testis. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: DEILAQAYTL LALEGLNQPS NESLQKVLQS GLKFVAARIP HLEPWRASLL LIWALTKLGG LSCCTTQLFA SSWGWQPPLI KSVPGSEPSK TQGQKRSGRG RQKLASAPLR LNNTSQKGLE GRGLPCTPKP PDRIRQAGPH VPFTVFEEVC PTESKPEVPQ APRVQQRVQT RLKVNFSDDS DLEDPVSAEA WLAEEPKRRG TASRGRGRAR KGLSLKTDAV VAPGSAPGNP GLNGRSRRAK KVASRHCEER RPQRASDQAR PGPEIMRTIP EEELTDNWRK M
靶标信息
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Caspase-like protein ESPL1; Cut1/ESP1 related protein; extra spindle pole bodies 1, separase; extra spindle pole bodies homolog 1; extra spindle poles like 1; Extra spindle poles-like 1 protein; FLJ46492; Separase; Separin; separin, cysteine protease
基因别名: AL024103; AU045071; Cerp; ESP1; ESPL1; KIAA0165; PRCE; SEPA; SSE
UniProt ID: (Human) Q14674, (Mouse) P60330
Entrez Gene ID: (Human) 9700, (Mouse) 105988, (Rat) 315330
mitotic sister chromatid segregation
meiotic spindle organization
cytokinesis
proteolysis
apoptotic process
establishment of mitotic spindle localization
homologous chromosome segregation
positive regulation of mitotic metaphase/anaphase transition
negative regulation of sister chromatid cohesion
meiotic chromosome separation
positive regulation of mitotic sister chromatid separation
chromosome segregation
mitotic nuclear division
meiosis I
