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Serpina12 Antibody (500-P256BT-25UG) in WB
Western Blot: To detect hVaspin by Western Blot analysis Serpina12 Polyclonal Antibody, Biotin (Product # 500-P256BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P256BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 45.2 kDa Recombinant Human Vaspin
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930102
产品详细信息
AA Sequence of recombinant protein: MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Vaspin. Anti-Human Vaspin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hVaspin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Vaspin (500-P256) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hVaspin.
Western Blot: To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P256BT-1MG will be provided as 2 x 500 µg
靶标信息
Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis of vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members. The serpins are irreversible ("suicidal") serine-protease inhibitors, characterized by having more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure, which contains an exposed reactive center loop that acts as a pseudo-substrate for the target proteinase. Members of this family play an important role in a number of fundamental biological processes, including blood coagulation, fibrinolysis, complement activation, angiogenesis, inflammation, and tumor suppression. In humans, the serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin. Yet, its protease inhibitory activity is still unknown. Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, reflected by normalized blood glucose levels. It also led to the reversal of altered expression of diabetes-relevant adipocytokines, including leptin, adiponectin, resistin, and TNF-alpha. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the diabetic/obesity metabolic syndrome.
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生物信息学
蛋白别名: a12; OL-64; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; Vaspin; Visceral adipose tissue-derived serine protease inhibitor; Visceral adipose-specific serpin
基因别名: OL-64; SERPINA12
UniProt ID: (Human) Q8IW75
Entrez Gene ID: (Human) 145264
glucose metabolic process
negative regulation of endopeptidase activity
positive regulation of phosphatidylinositol 3-kinase signaling
negative regulation of gluconeogenesis
positive regulation of insulin receptor signaling pathway
negative regulation of lipid biosynthetic process
regulation of cholesterol metabolic process
regulation of triglyceride metabolic process
