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Slit1 Antibody (600-401-860) in WB
Western blot using Rocklands Affinity Purified anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kDa. Approximately 20 µg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and ... View More
产品信息
600-401-860
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 485-515 of mouse SLIT-1 protein.
偶联物
形式
Liquid
浓度
1.2 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse SLIT-1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.
靶标信息
SLIT1 is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains 4; Multiple epidermal growth factor-like domains protein 4; Slit homolog 1 protein; Slit-1
基因别名: Kiaa0813; MEGF4; mKIAA0813; Slil1; Slit1
UniProt ID: (Rat) O88279, (Mouse) Q80TR4
Entrez Gene ID: (Rat) 65047, (Mouse) 20562
axonogenesis
axon guidance
brain development
motor neuron axon guidance
spinal cord development
olfactory bulb development
chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration
tangential migration from the subventricular zone to the olfactory bulb
telencephalon cell migration
retinal ganglion cell axon guidance
dorsal/ventral axon guidance
establishment of nucleus localization
neuron projection morphogenesis
negative regulation of axon extension involved in axon guidance
axon extension involved in axon guidance
negative chemotaxis
negative regulation of synapse assembly
multicellular organism development
nervous system development
cell differentiation
