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Smad1 Antibody (600-401-MN6) in ICC/IF
Immunofluorescence of Rabbit Anti-Smad1 Antibody. Cells: C2C12 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Smad1 at 10 µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5 µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Smad1 + DyLight™488. (C) Merge A+B. (D) Secondary Only. Expected localization: Nucleus, Plasma membrane, and Cytosol.
产品信息
600-401-MN6
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Smad1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 83% homology to human and rat.
靶标信息
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV5-1; MAD homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD5
基因别名: AI528653; Madh1; Madr1; Mlp1; MusMLP; Smad1
UniProt ID: (Mouse) P70340
Entrez Gene ID: (Mouse) 17125
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
mesodermal cell fate commitment
osteoblast fate commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
response to drug
homeostatic process
positive regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
positive regulation of cartilage development
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
