Invitrogen

Snail1 Monoclonal Antibody (20C8), Alexa Fluor™ 488, eBioscience™

Snail1 Antibody in Immunocytochemistry (ICC/IF)

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Snail1 Antibody (53-9859-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized MCF7 cells using 20 µg/mL Mouse IgG2a Alexa Fluor® 488 Isotype Control (left) or 20 µg/mL Anti-Human Snail1 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Snail1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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产品信息

53-9859-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

20 µg/mL

免疫细胞化学 (ICC/IF)

20 µg/mL

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

酶联免疫吸附实验 (ELISA)

Assay-Dependent

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

20C8

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574483

产品详细信息

Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.

Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, immunocytochemistry, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This 20C8 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and by immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma. This antibody can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunoprecipitation

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Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

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Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

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MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunocytochemistry

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MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

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EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

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HOXC10 promotes migration and invasion via the WNT-EMT signaling pathway in oral squamous cell carcinoma.

Journal of Cancer

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生物信息学

蛋白别名: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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基因别名: dJ710H13.1; SLUGH2; SNA; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863

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Entrez Gene ID: (Human) 6615

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功能

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding C2H2 zinc finger transcription factor

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