Invitrogen

Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody (ST0533)

Sodium Potassium ATPase Antibody in Immunocytochemistry (ICC/IF)

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Sodium Potassium ATPase Antibody (MA5-32184) in ICC/IF

Immunofluorescence analysis of ATP1A1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody (ST0533) (Product # MA5-32184) at 1:100 diluti... View More

Sodium Potassium ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Sodium Potassium ATPase Antibody in Western Blot (WB)

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产品信息

MA5-32184

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50,000-1:100,000

免疫组化（石蜡） (IHC (P))

1:2000-1:5,000

免疫细胞化学 (ICC/IF)

1:100-1:500

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ST0533

抗原

Synthetic peptide within Human ATP1A1 aa 39-83

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809472

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ptchd1 mediates opioid tolerance via cholesterol-dependent effects on μ-opioid receptor trafficking.

Nature neuroscience

Maza N,Wang D,Kowalski C,Stoveken HM,Dao M,Sial OK,Giles AC,Grill B,Martemyanov KA

MA5-32184 was used in Immunocytochemistry to propose that Ptchd1 plays an evolutionarily conserved role in protecting the µ-opioid receptor against overstimulation.

Thu Sep 01 00:00:00 UTC 2022

生物信息学

蛋白别名: ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+/K+ transporting, alpha 4 polypeptide; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; ATPase, Na+K+ transporting, alpha 1; Na(+)/K(+) ATPase alpha-1 subunit; Na(+)/K(+) ATPase alpha-4 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase alpha 4 subunit; Na+/K+ ATPase, alpha-D polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na,K-ATPase subunit alpha-C; sodium pump 4; Sodium pump subunit alpha-1; Sodium pump subunit alpha-4; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-4; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-4 chain; Sodium/potassium-transporting ATPase subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-4

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基因别名: ATP1A1; ATP1A4; ATP1AL2; Atpa-1; BC010319; Nkaa1b