Invitrogen

Sp56 Monoclonal Antibody (7C5)

Sp56 Antibody in Immunocytochemistry (ICC/IF)

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Sp56 Antibody (MA1-10866) in ICC/IF

Biology of reproduction 2015 - Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunocytochemistry

Sp56 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Sp56 Antibody — Fig 7 Chemical inhibition of GRASP55 affects spermatid differentiation in vivo. (A-B) Flow cytometry profiles of DAPI-stained germ cells isolated from testes of Gorasp2+/+ and Gorasp2-/- 35-days old mice (A) and 27-days old mice treated with vehicle or Graspin for two weeks (B). ES: elongated spermatids, RS: round spermatids, 2C: spermatogonia, 4C: primary spermatocytes. (C) Quantification of germ cell numbers of 27-days old mice treated with Graspin for two weeks and analyzed two days after the last Graspin injection. Vehicle, n = 10; Graspin, n = 10. Student’s unpaired t-test; *: P<0.05. (D) Confocal images of JAM-C, GRASP55 and DAPI staining of seminiferous tubule sections of 43-days old mice treated for 16 days with vehicle or Graspin. Differentiation stages of seminiferous tubules are indicated. High magnification pictures on the left panel highlight the loss of co-polarized localization of GRASP55 and JAM-C in developing round spermatids. Scale bars: left panels: 50 µm; right panels: 10 µm. (E) Quantification of co-polarized JAM-C and GRASP55 staining occurrence in control and treated mice expressed as the percentage of round spermatids showing a close apposition between GRASP55 and JAM-C stainings. Results obtained upon quantification of co-polarized staining occurrences in seminiferous tubules at stage V-VI (n = 415 counted events, n = 4 mice, 2 tissue sections/mice) and VIII (n = 498 counted events, n = 6 mice, 2 tissue sections/mice) are shown. Student’s unpaired t-test; **: P<0.01, ***: P<0.001. (F) Confocal images of SP56 and DAPI stainin {TM}

产品信息

MA1-10866

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-10 µg/mL

免疫组化 (IHC)

1-10 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

1-10 µg/mL

产品规格

种属反应

Hamster, Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

7C5

抗原

Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_1087440

产品详细信息

MA1-10866 detects mouse sperm protein sp56 from mouse and hamster samples. MA1-10866 does not cross-react with human sperm.

MA1-10866 has been successfully used in ELISA, Western blot, and immunohistochemistry applications. MA1-18066 will react with denatured, non-reduced sp56 but not with denatured, reduced sp56.

The MA1-10866 immunogen is a mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cylicins are a structural component of the sperm calyx being indispensable for male fertility in mice and human.

eLife

Schneider S,Kovacevic A,Mayer M,Dicke AK,Arévalo L,Koser SA,Hansen JN,Young S,Brenker C,Kliesch S,Wachten D,Kirfel G,Strünker T,Tüttelmann F,Schorle H

MA1-10866 was used in Immunohistochemistry to highlight the relevance and importance of Cylicins for spermiogenic remodeling and male fertility in human and mouse, and provides the basis for further studies on unraveling the complex molecular interactions between perinuclear theca proteins required during spermiogenesis.

Tue Nov 28 00:00:00 UTC 2023

Transient reduction of DNA methylation at the onset of meiosis in male mice.

Epigenetics & chromatin

Gaysinskaya V,Miller BF,De Luca C,van der Heijden GW,Hansen KD,Bortvin A

Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunofluorescence

Wed Apr 04 00:00:00 UTC 2018

Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis.

PLoS genetics

Cartier-Michaud A,Bailly AL,Betzi S,Shi X,Lissitzky JC,Zarubica A,Sergé A,Roche P,Lugari A,Hamon V,Bardin F,Derviaux C,Lembo F,Audebert S,Marchetto S,Durand B,Borg JP,Shi N,Morelli X,Aurrand-Lions M