Invitrogen

Spastin Polyclonal Antibody

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Spastin Antibody (PA5-53581) in WB

Western blot of SPAST was performed by loading 80 µg of WT (lanes 1/3) and SPAST CRISPR KO (lanes 2/4) HEK293T or HAP1 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. SPAST was detected at approximately 67 kDa (designated by the black arrow) using a SPAST polyclonal antibody (Product # PA5-53581) at a dilution of 1:500 in 5% BSA in TBS with 0.1% Tween 20 ... View More

Spastin Antibody in Immunoprecipitation (IP)

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产品信息

PA5-53581

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human Spastin. Recombinant protein control fragment (Product #RP-89991).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.10 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2647785

产品详细信息

Immunogen sequence: KRKDPLTHTS NSLPRSKTVM KTGSAGLSGH HRAPSYSGLS MVSGVKQGSG PAPTTHKGTP KTNRTNKPST PTTATRKKKD LKNFRNVDSN LANLIMNEIV DNGTAVKFDD IAGQDLAKQA LQEIVILPSL RPELFTGL

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.

靶标信息

This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。