Invitrogen

Syk Monoclonal Antibody (4D10.1), APC, eBioscience™

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Syk Antibody (17-6696-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) followed by intracellular staining with Mouse IgG2a K Isotype Control PE (Product # 17-4724-81) (left) or Anti-Human Syk APC (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-6696-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Rhesus monkey

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4D10.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10714836

产品详细信息

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.

Applications Reported: This 4D10.1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4D10.1 antibody has been pre-titrated and tested by intracellular staining using the Foxp3 buffer system and flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Specific human cytomegalovirus signature detected in NK cell metabolic changes post vaccination.

NPJ vaccines

Woods E,Zaiatz-Bittencourt V,Bannan C,Bergin C,Finlay DK,Hoffmann M,Brown A,Turner B,Makvandi-Nejad S,Vassilev V,Capone S,Folgori A,Hanke T,Barnes E,Dorrell L,Gardiner CM

Published figure using Syk monoclonal antibody (Product # 17-6696-42) in Flow Cytometry

Tue Sep 28 00:00:00 UTC 2021

CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Cell reports

Shah SV,Manickam C,Ram DR,Kroll K,Itell H,Permar SR,Barouch DH,Klatt NR,Reeves RK

17-6696-42 was used in Flow Cytometry to provide a mechanism of virus-dependent alternative signaling that may explain the acquisition of adaptive features by primate NK cells and could be targeted for future vaccine or curative therapies.

Tue Dec 04 00:00:00 UTC 2018

生物信息学

蛋白别名: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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基因别名: p72-Syk; SYK

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UniProt ID: (Human) P43405

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Entrez Gene ID: (Human) 6850

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功能

protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding

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