Invitrogen

Synaptophysin Monoclonal Antibody (SY38)

Synaptophysin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Synaptophysin Antibody (MA1-213) in IHC (PFA)

Immunohistochemistry was performed on human cerebellum and human breast tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 20 min. Following antigen retrieval, tissues were blocked in a 10% goat serum (Prod... View More

Synaptophysin Antibody in Western Blot (WB)

Synaptophysin Antibody in Flow Cytometry (Flow)

Synaptophysin Antibody in Immunohistochemistry (IHC)

Synaptophysin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

产品信息

MA1-213

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:1,500

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫组织化学（多聚甲醛固定） (IHC (PFA))

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100-1:500

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

1:200-1:500

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Guinea pig, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SY38

抗原

Synaptophysin from presynaptic vesicles from bovine brain

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2723681

产品详细信息

MA1-213 has been successfully used in western blot, imunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation and ELISA.

MA1-213 detects Synaptophysin in human, bovine, mouse and rat samples.

靶标信息

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

DOCK3 regulates normal skeletal muscle regeneration and glucose metabolism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Samani A,Karuppasamy M,English KG,Siler CA,Wang Y,Widrick JJ,Alexander MS

MA1-213 was used in Immunoprecipitation to demonstrate an essential role for DOCK3 in skeletal muscle independent of DOCK3 function in neuronal lineages.

Sun Oct 01 00:00:00 UTC 2023

Celf4 controls mRNA translation underlying synaptic development in the prenatal mammalian neocortex.

Nature communications

Salamon I,Park Y,Miškić T,Kopić J,Matteson P,Page NF,Roque A,McAuliffe GW,Favate J,Garcia-Forn M,Shah P,Judaš M,Millonig JH,Kostović I,De Rubeis S,Hart RP,Krsnik Ž,Rasin MR

MA1-213 was used in Immunohistochemistry to find that Celf4/CELF4-target mRNAs are encoded by risk genes for adverse neurodevelopmental outcomes translating into synaptic proteins.

Wed Sep 27 00:00:00 UTC 2023

Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.

The Journal of clinical investigation

Drumm MR,Wang W,Sears TK,Bell-Burdett K,Javier R,Cotton KY,Webb B,Byrne K,Unruh D,Thirunavu V,Walshon J,Steffens A,McCortney K,Lukas RV,Phillips JJ,Mohamed E,Finan JD,Santana-Santos L,Heimberger AB,Franz CK,Kurz J,Templer JW,Swanson GT,Horbinski C

MA1-213 was used in Immunocytochemistry to show that postoperative seizure risk in adult-type diffuse gliomas varies in large part by molecular subtype, and that IDHmut inhibitors could play a key role in mitigating such risk in IDHmut glioma patients.

Thu Jun 15 00:00:00 UTC 2023

Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.

The Journal of clinical investigation

Thu Jun 15 00:00:00 UTC 2023

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.

Nature communications

Wilson MN,Thunemann M,Liu X,Lu Y,Puppo F,Adams JW,Kim JH,Ramezani M,Pizzo DP,Djurovic S,Andreassen OA,Mansour AA,Gage FH,Muotri AR,Devor A,Kuzum D

MA1-213 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot to combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid.

Mon Dec 26 00:00:00 UTC 2022

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.

Nature communications

Wilson MN,Thunemann M,Liu X,Lu Y,Puppo F,Adams JW,Kim JH,Ramezani M,Pizzo DP,Djurovic S,Andreassen OA,Mansour AA,Gage FH,Muotri AR,Devor A,Kuzum D

Mon Dec 26 00:00:00 UTC 2022

Endothelial cells are an important source of BDNF in rat skeletal muscle.

Scientific reports

Cefis M,Chaney R,Quirié A,Santini C,Marie C,Garnier P,Prigent-Tessier A

MA1-213 was used in Immunocytochemistry to provide a comprehensive analysis of brain-derived neurotrophic factor localization in rat skeletal muscle.

Mon Jan 10 00:00:00 UTC 2022

Endothelial cells are an important source of BDNF in rat skeletal muscle.

Scientific reports

Cefis M,Chaney R,Quirié A,Santini C,Marie C,Garnier P,Prigent-Tessier A

MA1-213 was used in Immunocytochemistry to provide a comprehensive analysis of brain-derived neurotrophic factor localization in rat skeletal muscle.

Mon Jan 10 00:00:00 UTC 2022

Increased VLCFA-lipids and ELOVL4 underlie neurodegeneration in frontotemporal dementia.

Scientific reports

He Y,Phan K,Bhatia S,Pickford R,Fu Y,Yang Y,Hodges JR,Piguet O,Halliday GM,Kim WS

MA1-213 was used in Western Blot to investigate the role of very long chain fatty acids (VLCFA-lipids) in frontotemporal dementia.

Mon Nov 01 00:00:00 UTC 2021

Increased VLCFA-lipids and ELOVL4 underlie neurodegeneration in frontotemporal dementia.

Scientific reports

He Y,Phan K,Bhatia S,Pickford R,Fu Y,Yang Y,Hodges JR,Piguet O,Halliday GM,Kim WS

MA1-213 was used in Western Blot to investigate the role of very long chain fatty acids (VLCFA-lipids) in frontotemporal dementia.

Mon Nov 01 00:00:00 UTC 2021

Developmental Differences in Neocortex Neurogenesis and Maturation Between the Altricial Dwarf Rabbit and Precocial Guinea Pig.

Frontiers in neuroanatomy

Kalusa M,Heinrich MD,Sauerland C,Morawski M,Fietz SA

MA1-213 was used in Immunohistochemistry (PFA) to reveal important insights into the timing and cellular differences that regulate mammalian brain growth and maturation and provide a better understanding of the evolution of mammalian altriciality and presociality.