Invitrogen

Synaptotagmin 7 Monoclonal Antibody (N275/14)

Synaptotagmin 7 Antibody in Immunocytochemistry (ICC/IF)

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Synaptotagmin 7 Antibody (MA5-27654) in ICC/IF

Immunofluorescent analysis of Synaptotagmin 7 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Synaptotagmin 7 monoclonal antibody (Product # MA5-27654) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min a... View More

Synaptotagmin 7 Antibody in Western Blot (WB)

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产品信息

MA5-27654

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

N275/14

抗原

Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2735383

产品详细信息

1 µg/mL of MA5-27654 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approximately 65kD.

This antibody was formerly sold as clone S275-14.

靶标信息

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Beta cell dysfunction induced by bone morphogenetic protein (BMP)-2 is associated with histone modifications and decreased NeuroD1 chromatin binding.

Cell death & disease

Urizar AI,Prause M,Ingerslev LR,Wortham M,Sui Y,Sander M,Williams K,Barrès R,Larsen MR,Christensen GL,Billestrup N

MA5-27654 was used in Western Blotting to show that BMP-2 induces loss of beta cell maturity and suggest that remodeling of H3K27ac and decreased NeuroD1 DNA binding activity participate in the effect of BMP-2 on beta cell dysfunction.

Wed Jul 05 00:00:00 UTC 2023

Beta cell dysfunction induced by bone morphogenetic protein (BMP)-2 is associated with histone modifications and decreased NeuroD1 chromatin binding.

Cell death & disease

Urizar AI,Prause M,Ingerslev LR,Wortham M,Sui Y,Sander M,Williams K,Barrès R,Larsen MR,Christensen GL,Billestrup N

Wed Jul 05 00:00:00 UTC 2023

IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells.

Scientific reports

Hain A,Krämer M,Linka RM,Nakhaei-Rad S,Ahmadian MR,Häussinger D,Borkhardt A,Münk C

MA5-27654 was used in Immunocytochemistry-immunoflourescence to analyze the function of ITK during HIV-1 replication and showed that attachment, fusion of virions with the cell membrane and entry into Jurkat T-cells was inhibited when ITK was knocked down.