Invitrogen

Syndecan 4 Polyclonal Antibody

Syndecan 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Syndecan 4 Antibody (36-3100) in IHC (P)

Immunohistochemistry analysis of Syndecan-4 showing staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature... View More

Syndecan 4 Antibody in Western Blot (WB)

Syndecan 4 Antibody in Immunocytochemistry (ICC/IF)

Syndecan 4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

36-3100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

体外检测 (IV)

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产品规格

种属反应

Human

已发表种属

Human, Mammal, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the middle region of the human Syndecan-4 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533250

靶标信息

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan 1 is a matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan 1 and Syndecan 3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan 2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan 4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Altered shear stress on endothelial cells leads to remodeling of extracellular matrix and induction of angiogenesis.

PloS one

Russo TA,Banuth AMM,Nader HB,Dreyfuss JL

Published figure using Syndecan 4 polyclonal antibody (Product # 36-3100) in Immunocytochemistry

Mon Dec 21 00:00:00 UTC 2020

Syndecan-4 regulates extravillous trophoblast migration by coordinating protein kinase C activation.

Scientific reports

Jeyarajah MJ,Jaju Bhattad G,Kops BF,Renaud SJ

36-3100 was used in Immunohistochemistry to show that SDC4 has a role as a regulator of extravillous trophoblast motility, linked with coordination of PKCα activation.

Mon Jul 15 00:00:00 UTC 2019

Syndecan-4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition.

FEBS letters

Keller-Pinter A,Szabo K,Kocsis T,Deak F,Ocsovszki I,Zvara A,Puskas L,Szilak L,Dux L

36-3100 was used in Western Blotting to examine the regulation of myostatin activity by syndecan-4.

Sat Sep 01 00:00:00 UTC 2018

A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses.

Hepatology (Baltimore, Md.)

Verrier ER,Colpitts CC,Bach C,Heydmann L,Weiss A,Renaud M,Durand SC,Habersetzer F,Durantel D,Abou-Jaoudé G,López Ledesma MM,Felmlee DJ,Soumillon M,Croonenborghs T,Pochet N,Nassal M,Schuster C,Brino L,Sureau C,Zeisel MB,Baumert TF

36-3100 was used in in-vitro assays to identify glypican 5 as a common host cell entry factor for hepatitis B and delta viruses.

Fri Jan 01 00:00:00 UTC 2016

Syndecan-4 knockout leads to reduced extracellular transglutaminase-2 and protects against tubulointerstitial fibrosis.

Journal of the American Society of Nephrology : JASN

Scarpellini A,Huang L,Burhan I,Schroeder N,Funck M,Johnson TS,Verderio EA

Thu May 01 00:00:00 UTC 2014

Syndecan-4 knockout leads to reduced extracellular transglutaminase-2 and protects against tubulointerstitial fibrosis.

Journal of the American Society of Nephrology : JASN

Scarpellini A,Huang L,Burhan I,Schroeder N,Funck M,Johnson TS,Verderio EA

Thu May 01 00:00:00 UTC 2014

Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.

Journal of virology

Kalia M,Chandra V,Rahman SA,Sehgal D,Jameel S

Tue Dec 01 00:00:00 UTC 2009

Hypoxia increases macrophage motility, possibly by decreasing the heparan sulfate proteoglycan biosynthesis.

Journal of leukocyte biology

Asplund A,Ostergren-Lundén G,Camejo G,Stillemark-Billton P,Bondjers G

Sat Aug 01 00:00:00 UTC 2009

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

生物信息学

蛋白别名: Amphiglycan; MGC22217; ryudocan amphiglycan; Ryudocan core protein; SYND4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4

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基因别名: SDC4; SYND4

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UniProt ID: (Human) P31431

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Entrez Gene ID: (Human) 6385

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功能

fibronectin binding protein kinase C binding protein binding thrombospondin receptor activity transmembrane signal receptor

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