Invitrogen

Syndecan 4 Recombinant Rabbit Monoclonal Antibody (112)

Syndecan 4 Antibody in Western Blot (WB)

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Syndecan 4 Antibody (MA5-29784) in WB

Western Blot using Syndecan 4 Recombinant Rabbit Monoclonal Antibody (112) (Product # MA5-29784) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa.

Syndecan 4 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-29784

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

流式细胞分析 (Flow)

1:25-1:100

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

112

抗原

Recombinant Mouse Syndecan-4/SDC4 protein (Met1-Val146)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2785606

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Mouse Syndecan-4/SDC4.

靶标信息

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan 1 is a matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan 1 and Syndecan 3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan 2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan 4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。