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TAF1 Antibody (PA5-104490) in ICC/IF
Immunofluorescent analysis of TAF1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TAF1 polyclonal antibody (Product # PA5-104490) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-104490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAF1(Accession P21675), corresponding to amino acid residues R1146-R1196.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853791
Product Specific Information
Antibody detects endogenous levels of total TAF1.
Target Information
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle gene 1 protein; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; p250; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, neuron specific isoform; TATA box binding protein associated factor 1; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; Transcription initiation factor TFIID 250 kDa subunit; Transcription initiation factor TFIID subunit 1
Gene Aliases: AU015687; B430306D02Rik; BA2R; Ccg-1; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; RGD1562050; TAF(II)250; TAF1; TAF2A; TAFII-250; TAFII250; XDP
UniProt ID: (Human) P21675, (Mouse) Q80UV9
Entrez Gene ID: (Human) 6872, (Mouse) 270627, (Rat) 317256
p53 binding
transcription coactivator activity
histone acetyltransferase activity
protein serine/threonine kinase activity
protein binding
ATP binding
transcription factor binding
TBP-class protein binding
sequence-specific DNA binding
transcription regulatory region DNA binding
lysine-acetylated histone binding
nucleotide binding
DNA binding
kinase activity
transferase activity
transferase activity, transferring acyl groups
molecular_function
general transcription factor
DNA-templated transcription, initiation
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell cycle
viral process
histone acetylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
regulation of signal transduction by p53 class mediator
transcription, DNA-templated
regulation of transcription, DNA-templated
phosphorylation
response to drug
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