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TAF12 Antibody (PA5-106322) in ICC/IF
Immunofluorescent analysis of TAF12 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TAF12 polyclonal antibody (Product # PA5-106322) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-106322
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TAF12(Accession Q16514), corresponding to amino acid residues M1-S51.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853999
产品详细信息
Antibody detects endogenous levels of total TAF12.
靶标信息
TAF12 is involved in the control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20 kDa; TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa; TAFII-20; TAFII-20/TAFII-15; TAFII20; TAFII20/TAFII15; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD; TATA box binding protein associated factor 12; Transcription initiation factor TFIID 20 kDa subunits; Transcription initiation factor TFIID 20/15 kDa subunits; Transcription initiation factor TFIID subunit 12
基因别名: 20kDa; 2810422D08Rik; AW557038; TAF12; TAF15; TAF2J; TAFII20
UniProt ID: (Human) Q16514, (Mouse) Q8VE65
Entrez Gene ID: (Human) 6883, (Mouse) 66464, (Rat) 682902
DNA-templated transcription, initiation
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of signal transduction by p53 class mediator
transcription, DNA-templated
regulation of transcription, DNA-templated
histone H3 acetylation
regulation of nucleic acid-templated transcription
biological_process
