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FIGURE: 1 / 9
TAF7 Antibody (PA5-114783) in ICC/IF
Immunocytochemistry analysis of TAF7 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114783). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-114783
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAF7(Accession Q15545), corresponding to amino acid residues R180-S230.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899419
Product Specific Information
Antibody detects endogenous levels of total TAF7.
Target Information
The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II. The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA polymerase II TBP-associated factor subunit F; TAF(II)55; TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55 kDa; TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa; TAFII-55; TATA box binding protein (TBP) associated factor, RNA polymerase II, F, 55 kDa; TATA box binding protein (TBP)-associated factor, RNA polymerase II, F, 55kD; TATA box binding protein associated factor 7; TBP-associated factor F; transcription factor IID subunit TAFII55; Transcription initiation factor TFIID 55 kDa subunit; Transcription initiation factor TFIID subunit 7; transcription initiation factor TFIID, 55 kDa subunit
Gene Aliases: 55kDa; AI607308; BB007175; TAF2F; TAF7; TAFII55
UniProt ID: (Human) Q15545, (Mouse) Q9R1C0
Entrez Gene ID: (Human) 6879, (Rat) 307485, (Mouse) 24074
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
histone acetyltransferase binding
vitamin D receptor binding
transcription regulatory region DNA binding
thyroid hormone receptor binding
molecular_function
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
spermine transport
DNA-templated transcription, initiation
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
negative regulation of protein kinase activity
intracellular estrogen receptor signaling pathway
negative regulation of histone acetylation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
regulation of transcription from RNA polymerase II promoter
biological_process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of nucleic acid-templated transcription
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