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TAS2R9 Antibody (PA5-99932) in ICC/IF
Immunocytochemistry analysis of TAS2R9 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TAS2R9 polyclonal antibody (Product # PA5-99932) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-99932
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TAS2R9(Accession Q9NYW1), corresponding to amino acid residues L124-I174.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818865
产品详细信息
Antibody detects endogenous levels of total TAS2R9.
靶标信息
This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13. Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T2R9; Taste receptor family B member 6; Taste receptor type 2 member 9; taste receptor, family B, member 6; taste receptor, type 2, member 9; TRB6
基因别名: T2R9; TAS2R9; TRB6
UniProt ID: (Human) Q9NYW1
Entrez Gene ID: (Human) 50835
