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TBCE Antibody (PA5-100346) in ICC/IF
Immunofluorescent analysis of TBCE in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TBCE polyclonal antibody (Product # PA5-100346) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100346
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TBCE(Accession Q15813), corresponding to amino acid residues R24-K74.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2849859
产品详细信息
Antibody detects endogenous levels of total TBCE.
靶标信息
Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hypoparathyroidism, growth and mental retardation, and dysmorphism; Tubulin-folding cofactor E; Tubulin-specific chaperone E
基因别名: 2610206D02Rik; C530005D02Rik; HRD; KCS; KCS1; pac2; pmn; TBCE
UniProt ID: (Human) Q15813, (Rat) Q5FVQ9, (Mouse) Q8CIV8
Entrez Gene ID: (Human) 6905, (Rat) 361255, (Mouse) 70430
