Invitrogen

TBK1 Polyclonal Antibody

TBK1 Antibody in Immunocytochemistry (ICC/IF)

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TBK1 Antibody (PA5-17478) in ICC/IF

Immunofluorescence analysis of TBK1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TBK1 Polyclonal Antibody (Product # PA5-17478) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # PA5-17478) at... View More

TBK1 Antibody in Immunohistochemistry (IHC)

TBK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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TBK1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of the target protein. A loss of signal was observed for the target protein in the TBK1 KO cell line compared to the control cell line using an Anti-TBK1 Polyclonal Antibody (Product # PA5-17478). {KO}

产品信息

PA5-17478

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:100

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues near the carboxy-terminus of TBK1/NAK

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

174 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10981817

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

TBK1 serine/threonine kinase is a member of the IKK family and is involved in NF-kappa B activation and IRF3 signaling. TBK1 is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKK beta, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKK beta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

PA5-17478 was used in Western Blotting to identify antibodies that appear specific for all three applications but invite the readers to interpret the present findings based on their own scientific expertise and use this report as a guide to select the most appropriate antibody for their specific needs.

Wed Dec 07 00:00:00 UTC 2022

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

Wed Dec 07 00:00:00 UTC 2022

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research

Alshafie W,Fotouhi M,Shlaifer I,Ayoubi R,Edwards AM,Durcan TM,McPherson PS,Laflamme C

Wed Dec 07 00:00:00 UTC 2022

Bacterial Cyclic Dinucleotides and the cGAS-cGAMP-STING Pathway: A Role in Periodontitis?

Pathogens (Basel, Switzerland)

Elmanfi S,Yilmaz M,Ong WWS,Yeboah KS,Sintim HO,Gürsoy M,Könönen E,Gürsoy UK

Published figure using TBK1 polyclonal antibody (Product # PA5-17478) in Immunohistochemistry

Sun May 30 00:00:00 UTC 2021

Association of the TBK1 mutation p.Ile334Thr with frontotemporal dementia and literature review.

Molecular genetics & genomic medicine

Yu H,Yu W,Luo SS,Yang YJ,Liu FT,Zhang Y,Chen Y,Sun YM,Wu JJ

Published figure using TBK1 polyclonal antibody (Product # PA5-17478) in Western Blot

Fri Mar 01 00:00:00 UTC 2019

Haploinsufficiency of TBK1 causes familial ALS and fronto-temporal dementia.

Nature neuroscience

Freischmidt A,Wieland T,Richter B,Ruf W,Schaeffer V,Müller K,Marroquin N,Nordin F,Hübers A,Weydt P,Pinto S,Press R,Millecamps S,Molko N,Bernard E,Desnuelle C,Soriani MH,Dorst J,Graf E,Nordström U,Feiler MS,Putz S,Boeckers TM,Meyer T,Winkler AS,Winkelman J,de Carvalho M,Thal DR,Otto M,Brännström T,Volk AE,Kursula P,Danzer KM,Lichtner P,Dikic I,Meitinger T,Ludolph AC,Strom TM,Andersen PM,Weishaupt JH

PA5-17478 was used in western blot to identify gene variants that contribute to amyotrophic lateral sclerosis

Fri May 01 00:00:00 UTC 2015

生物信息学

蛋白别名: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk

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基因别名: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1

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UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2

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Entrez Gene ID: (Human) 29110, (Mouse) 56480, (Rat) 299827

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功能