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TBX3 Antibody (711087) in ICC/IF
For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous TBX3 using Anti- TBX3 Recombinant Rabbit Superclonal™ Antibody (Product # 711087, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TBX3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
产品信息
711087
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
22HCLC
抗原
Protein corresponding to Human TBX3 (aa 305-500)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633115
产品详细信息
This antibody is predicted to react with Monkey, Pig and Rat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: bladder cancer related protein XHL; T-box protein 3; T-box transcription factor TBX3
基因别名: TBX3; TBX3-ISO; UMS; XHL
UniProt ID: (Human) O15119
Entrez Gene ID: (Human) 6926
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
protein binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
blood vessel development
in utero embryonic development
heart looping
outflow tract morphogenesis
atrioventricular bundle cell differentiation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell aging
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
organ morphogenesis
specification of organ position
stem cell population maintenance
limbic system development
male genitalia development
female genitalia development
negative regulation of epithelial cell differentiation
mammary gland development
luteinizing hormone secretion
embryonic forelimb morphogenesis
embryonic hindlimb morphogenesis
forelimb morphogenesis
embryonic digit morphogenesis
negative regulation of apoptotic process
negative regulation of myoblast differentiation
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
follicle-stimulating hormone secretion
mesoderm morphogenesis
palate development
ventricular septum morphogenesis
branching involved in mammary gland duct morphogenesis
mammary placode formation
cardiac muscle cell fate commitment
sinoatrial node cell development
cellular senescence
positive regulation of stem cell proliferation
