Invitrogen

TBX5 Polyclonal Antibody

TBX5 Antibody in Immunocytochemistry (ICC/IF)

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TBX5 Antibody (42-6500) in ICC/IF

Immunofluorescence analysis of TBX5 Polyclonal Antibody was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TBX5 Rabbit Polyclonal Antibody (Product # 42-6500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conju... View More

TBX5 Antibody in Immunohistochemistry, Immunoprecipitation (IHC, IP)

TBX5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

TBX5 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

42-6500

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from an internal region of the human, predicted dog, and predicted chimpanzee Tbx5 proteins, which differ from mouse and rat by one conservative amino acid change

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533533

靶标信息

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.

Biology open

Dai Y,Nasehi F,Winchester CD,Foley AC

42-6500 was used in Immunocytochemistry to understand the role of Tbx5 in sinoatrial node differentiation.

Thu Jun 15 00:00:00 UTC 2023

Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.

Biology open

Dai Y,Nasehi F,Winchester CD,Foley AC

42-6500 was used in Immunocytochemistry to understand the role of Tbx5 in sinoatrial node differentiation.

Thu Jun 15 00:00:00 UTC 2023

Generation of left ventricle-like cardiomyocytes with improved structural, functional, and metabolic maturity from human pluripotent stem cells.

Cell reports methods

Dark N,Cosson MV,Tsansizi LI,Owen TJ,Ferraro E,Francis AJ,Tsai S,Bouissou C,Weston A,Collinson L,Abi-Gerges N,Miller PE,MacLeod KT,Ehler E,Mitter R,Harding SE,Smith JC,Bernardo AS

42-6500 was used in Immunocytochemistry to show that functionally matured hPSC-LV-CMs can be obtained rapidly without exposure to current maturation regimes.

Mon Apr 24 00:00:00 UTC 2023

Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury.

Frontiers in cell and developmental biology

Zhao H,Zhang Y,Xu X,Sun Q,Yang C,Wang H,Yang J,Yang Y,Yang X,Liu Y,Zhao Y

42-6500 was used in Immunocytochemistry to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases.

Tue Sep 19 00:00:00 UTC 2023

Identification and analysis of KLF13 variants in patients with congenital heart disease.

BMC medical genetics

Li W,Li B,Li T,Zhang E,Wang Q,Chen S,Sun K

42-6500 was used in Western Blotting to aime to screen the KLF13 variants in CHD patients and genetically analyze the functions of these variants.

Wed Apr 15 00:00:00 UTC 2020

Identification and analysis of KLF13 variants in patients with congenital heart disease.

BMC medical genetics

Li W,Li B,Li T,Zhang E,Wang Q,Chen S,Sun K

42-6500 was used in Western Blotting to aime to screen the KLF13 variants in CHD patients and genetically analyze the functions of these variants.

Wed Apr 15 00:00:00 UTC 2020

Identification and analysis of KLF13 variants in patients with congenital heart disease.

BMC medical genetics

Li W,Li B,Li T,Zhang E,Wang Q,Chen S,Sun K

42-6500 was used in Western Blotting to aime to screen the KLF13 variants in CHD patients and genetically analyze the functions of these variants.

Wed Apr 15 00:00:00 UTC 2020

Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development.

Biochimica et biophysica acta

Jeong HS,Jung ES,Sim YJ,Kim SJ,Jang JW,Hong KS,Lee WY,Chung HM,Park KT,Jung YS,Kim CH,Kim KS

42-6500 was used in immunocytochemistry to determine the molecular mechanisms underlying Fbxo25-mediated ubiquitination of cardiac transcription factors

Mon Jun 01 00:00:00 UTC 2015

Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte proliferation and atrioventricular septation.

Human molecular genetics