Invitrogen

TBX6 Polyclonal Antibody

TBX6 Antibody in Immunocytochemistry (ICC/IF)

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TBX6 Antibody (PA5-35102) in ICC/IF

Immunocytochemistry analysis of TBX6 in HeLa cells. Samples were incubated with TBX6 polyclonal antibody (Product # PA5-35102) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). TBX6 im... View More

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产品信息

PA5-35102

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:10-1:50

查看9篇已发表文章 9篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 264-293 amino acids from the central region of human TBX6

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2552412

产品详细信息

This antibody is predicted to react with bovine and rat based on sequence homology.

靶标信息

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients.

Stem cell research

Zushin PH,Zhou Y,Li A,Ashley EA,Wheeler MT,Wu JC

PA5-35102 was used in Immunocytochemistry to generate two human iPSC lines from individuals with mutations in exon 3 of BAG3 and provide validation of their pluripotency and ability to differentiate toward the three primary germ layers.

Wed Mar 01 00:00:00 UTC 2023

Generation of two iPSC lines from long QT syndrome patients carrying SNTA1 variants.

Stem cell research

Jimenez-Tellez N,Vera CD,Yildirim Z,Vicente Guevara J,Zhang T,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to generate two human-induced pluripotent stem cell (iPSC) lines from two LQT patients carrying a missense mutation in SNTA1 (c.1088A > C).

Wed Feb 01 00:00:00 UTC 2023

Generation of human induced pluripotent stem cell lines carrying heterozygous PLN mutation from dilated cardiomyopathy patients.

Stem cell research

Caudal A,Mondejar-Parreño G,Vera CD,Williams DR,Shenoy SP,Liang D,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to show that these patient-specific iPSC lines serve as valuable in vitro models for DCM pathology caused by PLN mutations.

Mon Aug 01 00:00:00 UTC 2022

Generation of human induced pluripotent stem cell lines carrying heterozygous PLN mutation from dilated cardiomyopathy patients.

Stem cell research

Caudal A,Mondejar-Parreño G,Vera CD,Williams DR,Shenoy SP,Liang D,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to show that these patient-specific iPSC lines serve as valuable in vitro models for DCM pathology caused by PLN mutations.

Mon Aug 01 00:00:00 UTC 2022

Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants.

Stem cell research

Manhas A,Jahng JWS,Vera CD,Shenoy SP,Knowles JW,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to show that both iPSC lines expressed pluripotent markers, had a normal karyotype, and were able to differentiate into three germ layers, making them potentially valuable tools for modeling HCM in vitro and investigating the pathological mechanisms related to these two variants.

Sun May 01 00:00:00 UTC 2022

Generation of two induced pluripotent stem cell lines carrying the phospholamban R14del mutation for modeling ARVD/C.

Stem cell research

Vera CD,Manhas A,Shenoy SP,Wheeler MT,Sallam K,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to show two lines carrying this mutation with typical iPSC morphology, pluripotency, karyotype, ability to differentiate into the three germ layers in vitro, and readily availability for studying pathological mechanisms or ARVD/C. Copyright © 2022.

Mon Aug 01 00:00:00 UTC 2022

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene.

Stem cell research

Yildirim Z,Kojic A,Yan CD,Wu MA,Vagelos R,Wu JC

PA5-35102 was used in Immunocytochemistry-immunofluorescence to generate two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A).

Thu Dec 01 00:00:00 UTC 2022

Generation of three induced pluripotent stem cell lines to model and investigate diseases affecting Hispanics.

Stem cell research

Chen IY,Olshausen J,Thomas D,Lai C,McLaughlin TL,Wu JC

PA5-35102 was used in Immunocytochemistry to generate three induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of healthy Hispanic subjects.

Thu Dec 01 00:00:00 UTC 2022

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).

Stem cell research

Alonzo M,Contreras J,Ye S,Lin H,Hernandez-Rosario L,McBride KL,Texter K,Garg V,Zhao MT

PA5-35102 was used in Immunocytochemistry-immunofluorescence to generante and validate an iPSC line from the peripheral blood mononuclear cells of a male patient with hypoplastic left heart syndrome (HLHS) through Sendai virus-mediated transfection of 4 Yamanaka factors.

Sat Oct 01 00:00:00 UTC 2022

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).

Stem cell research

Alonzo M,Contreras J,Ye S,Lin H,Hernandez-Rosario L,McBride KL,Texter K,Garg V,Zhao MT

Sat Oct 01 00:00:00 UTC 2022

Generation of three induced pluripotent stem cell lines (SCVIi014-A, SCVIi015-A, and SCVIi016-A) from patients with LQT1 caused by heterozygous mutations in the KCNQ1 gene.

Stem cell research

Zhang H,Jahng JWS,Liu Y,Chase AJ,Perez MV,Wu JC

PA5-35102 was used in Immunocytochemistry to generated three human-induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMCs) of LQT1 patients carrying pathogenic variants (c.569 G>A, c.585delG, and c.573_577delGCGCT) in KCNQ1.