Invitrogen

TCF21 Polyclonal Antibody

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抗体检测数据 (2)

TCF21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TCF21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TCF21 Antibody (PA5-119812) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse brain tissue using TCF21 Polyclonal Antibody (Product # PA5-119812). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the TCF21 antibody at a dilution of ... View More

TCF21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TCF21 Antibody in Western Blot (WB)

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TCF21 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse brain tissue using TCF21 Polyclonal Antibody (Product # PA5-119812). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the TCF21 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

TCF21 Antibody in Western Blot (WB) — Western blot analysis of TCF21 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. TCF21 Polyclonal Antibody (Product # PA5-119812) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate. Lane 2: K562 cell lysate. Lane 3: Hela cell lysate. Lane 4: Human lung tissue lysate. Lane 5: Mouse kidney tissue lysate.

产品信息

PA5-119812

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within human TCF21 aa 52-90

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913384

产品详细信息

Positive Control: 293T cell lysate, K562 cell lysate, Hela cell lysate, human lung tissue lysate, mouse kidney tissue lysate, mouse brain tissue.

Subcellular Location: Nucleus.

靶标信息

The basic helix-loop-helix transcription factor TCF21/Pod1 (also called capsulin or epicardin) is involved in kidney, lung and spleen organogenesis. It is also essential for normal development of the testes and ovaries. TCF21/Pod1 is involved in the transcriptional repression of steroidogenic factor 1 (Sf1/Nr5a1/Ad4BP), an orphan nuclear receptor that regulates the expression of multiple genes (including Scc) that mediate sexual differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: bHLHa23; Capsulin; Class A basic helix-loop-helix protein 23; Epicardin; Pod-1; Podocyte-expressed 1; TCF-21; Transcription factor 21

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基因别名: BHLHA23; capsulin; epc; epicardin; Pod-1; POD1; TCF21

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UniProt ID: (Human) O43680, (Mouse) O35437

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Entrez Gene ID: (Human) 6943, (Mouse) 21412

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transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding histone deacetylase binding bHLH transcription factor binding protein dimerization activity androgen receptor binding E-box binding DNA binding protein binding basic helix-loop-helix transcription factor

negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis morphogenesis of a branching structure kidney development vasculature development transcription from RNA polymerase II promoter sex determination branchiomeric skeletal muscle development epithelial cell differentiation glomerulus development positive regulation of transcription from RNA polymerase II promoter lung alveolus development spleen development embryonic digestive tract morphogenesis reproductive structure development gland development Sertoli cell differentiation palate development lung morphogenesis lung vasculature development bronchiole development diaphragm development respiratory system development negative regulation of androgen receptor signaling pathway metanephric mesenchymal cell differentiation metanephric glomerular capillary formation transcription, DNA-templated regulation of transcription, DNA-templated sex differentiation organ morphogenesis regulation of histone deacetylation