Invitrogen

TCF7 Monoclonal Antibody (C.725.7)

TCF7 Antibody in Immunocytochemistry (ICC/IF)

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TCF7 Antibody (MA5-14965) in ICC/IF

Immunofluorescent analysis of TCF1 in DLD-1 cells using a TCF1 monoclonal antibody (Product # MA5-14965) (green). Actin filaments are labeled with a fluorescent red phalloidin.

TCF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TCF7 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

TCF7 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of TCF7 was observed in MOLT-4, Jurkat, HeLa, SW480 in comparison to U2OS and LNCap and in Mouse Thymus as compared to Mouse Skeletal Muscle using Anti-TCF7 Monoclonal Antibody (C.725.7) (Product # MA5-14965) in Western Blot. {RE}

产品信息

MA5-14965

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:200-1:800

流式细胞分析 (Flow)

1:50-1:200

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

1:50

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

C.725.7

抗原

Synthetic peptide corresponding to a region surrounding Pro96 of human TCF1 protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

100 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10983684

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with LEF1.

靶标信息

TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer.

Nature communications

Omatsu M,Nakanishi Y,Iwane K,Aoyama N,Duran A,Muta Y,Martinez-Ordoñez A,Han Q,Agatsuma N,Mizukoshi K,Kawai M,Yamakawa G,Namikawa M,Hamada K,Fukunaga Y,Utsumi T,Sono M,Masuda T,Hata A,Araki O,Nagao M,Yoshikawa T,Ogawa S,Hiramatsu Y,Tsuda M,Maruno T,Kogame T,Kasashima H,Kakiuchi N,Nakagawa MM,Kawada K,Yashiro M,Maeda K,Saito Y,Matozaki T,Fukuda A,Kabashima K,Obama K,Ogawa S,Sheibani N,Diaz-Meco MT,Moscat J,Seno H

MA5-14965 was used in Immunohistochemistry to establish THBS1 as a potential biomarker for identifying mesenchymal CRC and as a critical suppressor of antitumor immunity that contributes to the progression of this malignancy with a poor prognosis.

Mon Sep 25 00:00:00 UTC 2023

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

MA5-14965 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 UTC 2022

Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires β-catenin activity to drive T-cell acute lymphoblastic leukemia.

Nature communications

Van Thillo Q,De Bie J,Seneviratne JA,Demeyer S,Omari S,Balachandran A,Zhai V,Tam WL,Sweron B,Geerdens E,Gielen O,Provost S,Segers H,Boeckx N,Marshall GM,Cheung BB,Isobe K,Kato I,Takita J,Amos TG,Deveson IW,McCalmont H,Lock RB,Oxley EP,Garwood MM,Dickins RA,Uyttebroeck A,Carter DR,Cools J,de Bock CE

MA5-14965 was used in Western Blot to show that targeting the TCF7-SPI1 fusion in vivo with a doxycycline-inducible knockdown results in increased differentiation.

Tue Jul 06 00:00:00 UTC 2021

Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires β-catenin activity to drive T-cell acute lymphoblastic leukemia.

Nature communications

MA5-14965 was used in Western Blot to show that targeting the TCF7-SPI1 fusion in vivo with a doxycycline-inducible knockdown results in increased differentiation.

Tue Jul 06 00:00:00 UTC 2021

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

MA5-14965 was used in Immunohistochemistry to investigate the effect of prolonged expression of transgenic p16INK4a in the mouse epidermis and the pathways involved in this effect.

Mon Jun 01 00:00:00 UTC 2020

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

MA5-14965 was used in Immunohistochemistry to investigate the effect of prolonged expression of transgenic p16INK4a in the mouse epidermis and the pathways involved in this effect.

Mon Jun 01 00:00:00 UTC 2020

Intrinsic 4-1BB signals are indispensable for the establishment of an influenza-specific tissue-resident memory CD8 T-cell population in the lung.

Mucosal immunology

Zhou AC,Wagar LE,Wortzman ME,Watts TH

MA5-14965 was used in Flow cytometry/Cell sorting to show that intranasal administration of 4-1BBL along with influenza nucleoprotein in a replicationdefective adenovirus vector to influenza pre-immune mice induces a remarkably stable circulating effector memory CD8 T-cell population.

Fri Sep 01 00:00:00 UTC 2017

β-catenin is required for taste bud cell renewal and behavioral taste perception in adult mice.

PLoS genetics

Gaillard D,Bowles SG,Salcedo E,Xu M,Millar SE,Barlow LA

MA5-14965 was used in Immunohistochemistry to show that conditional β-catenin deletion in mouse taste progenitors leads to rapid depletion of progenitors and Shh+ precursors, which in turn causes taste bud loss, followed by loss of gustatory nerve fibers.

Tue Aug 01 00:00:00 UTC 2017

β-catenin is required for taste bud cell renewal and behavioral taste perception in adult mice.

PLoS genetics

Gaillard D,Bowles SG,Salcedo E,Xu M,Millar SE,Barlow LA

Tue Aug 01 00:00:00 UTC 2017

生物信息学

蛋白别名: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T cell factor-1; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7; transcription factor 7, T-cell specific

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基因别名: AI465550; TCF-1; TCF1; TCF7

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UniProt ID: (Human) P36402, (Mouse) Q00417

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Entrez Gene ID: (Human) 6932, (Mouse) 21414

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功能