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TCR V beta 2 Antibody (11-5804-82) in Flow
Staining of C57Bl/6 lymph node cells with Anti-Mouse CD4 APC (Product # 17-0041-82) with Anti-Mouse CD6 APC (Product # 17-0081-82) and 0.5 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.5 µg of Anti-Mouse V beta 2 TCR FITC (right). Total viable cells were used for analysis.
产品信息
11-5804-82
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
B20.6
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10718838
产品详细信息
Description: The B20.6 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 2 chain. Neonatal T cells which express V beta 2, and are exposed to the Balb/cV virus are eliminated during negative selection, while adult T cells expressing V beta 2 and exposed to the Balb/cV virus are clonally expanded.
Applications Reported: This B20.6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B20.6 antibody has been tested by flow cytometric analysis of lymph node cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: TCR Vb2; TCRV beta-2; Vb 2; Vb2; Vbeta 2; Vbeta2
