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TCR gamma/delta Antibody (11-5810-81) in Flow
Staining of rat splenocytes with Anti-Rat CD5 APC (Product # 17-0050-82) and 0.125 µg of Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or 0.125 µg of Anti-Rat gamma delta TCR FITC (right). Cells in the lymphocyte gate were used for analysis.
产品信息
11-5810-81
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
V65
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_465253
产品详细信息
Description: The V65 monoclonal antibody reacts with the gamma/delta chain of rat TCR. gammadelta TCR is expressed by dendritic epidermal T cells. Cross-linking of the TCR complex with V65 induces cytokine-dependent T cell stimulation.
Applications Reported: The V65 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The V65 antibody has been tested by flow cytometric analysis of rat splenocytes and lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex
基因别名: Tcrg; Trg@
Entrez Gene ID: (Rat) 24821
