Invitrogen

TDG Monoclonal Antibody (GT622)

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TDG Antibody (MA5-31519) in WB

Western blot analysis of TDG in 293T mock transfected (-) or transfected with a construct expressing human TDG using TDG monoclonal antibody (Product # MA5-31519) at a dilution of 1:10000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

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产品信息

MA5-31519

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

GT622

抗原

Recombinant protein encompassing a sequence within the center region of human TDG.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2787150

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).

Positive Control: human TDG-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

仅用于科研。不用于诊断过程。未经明确授权不得转售。