Invitrogen

TDP-43 Recombinant Superclonal™ Antibody (1HCLC)

TDP-43 Antibody in Immunocytochemistry (ICC/IF)

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TDP-43 Antibody (711051) in ICC/IF

Immunofluorescence analysis of TDP-43 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TDP-43 Recombinant Rabbit Superclonal™ Antibody (1HCLC) (Product # 711051) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More

TDP-43 Antibody in Immunoprecipitation (IP)

TDP-43 Antibody in RNA Immunoprecipitation (RIP)

TDP-43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

711051

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

RNA免疫共沉淀 (RIP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

1HCLC

抗原

Protein corresponding to Human TARDBP (aa 130-275)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2633110

产品详细信息

This antibody is predicted to react with Monkey, Bovine and Horse

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

711051 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

711051 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

711051 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

711051 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

TDP-43 as structure-based biomarker in amyotrophic lateral sclerosis.

Annals of clinical and translational neurology

Beyer L,Günther R,Koch JC,Klebe S,Hagenacker T,Lingor P,Biesalski AS,Hermann A,Nabers A,Gold R,Tönges L,Gerwert K

711051 was used in Western Blot to analyse the secondary structure distribution of TDP-43 in cerebrospinal fluid of ALS patients (n = 36) compared to Parkinson´s disease patients (PD; n = 30) and further controls (Ctrl; n = 24) using the immuno-infrared sensor technology.