Invitrogen

TDP-43 Recombinant Rabbit Monoclonal Antibody (JM51-10)

TDP-43 Antibody in Immunocytochemistry (ICC/IF)

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TDP-43 Antibody (MA5-32627) in ICC/IF

Immunofluorescence of TDP-43 was performed using HAP1 wild-type and TARDBP KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the TDP-43 recombinant mo... View More

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TDP-43 Antibody in Flow Cytometry (Flow)

TDP-43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

MA5-32627

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫组化（石蜡） (IHC (P))

1:500

免疫细胞化学 (ICC/IF)

1:250-1:500

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流式细胞分析 (Flow)

1:50-1:100

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat, Zebrafish

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM51-10

抗原

Synthetic peptide within Human TDP43 aa 354-390

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809904

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

仅用于科研。不用于诊断过程。未经明确授权不得转售。