Invitrogen

TEK Polyclonal Antibody

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TEK Antibody (PA5-28582) in WB

Western Blot using TEK Polyclonal Antibody (Product # PA5-28582). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with TEK Polyclonal Antibody (Product # PA5-28582) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TEK Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-28582

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

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in situ PLA (PLA)

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产品规格

种属反应

Human

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 337 of Human TIE2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.75 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546058

产品详细信息

Recommended positive controls: HUVEC.

Predicted reactivity: Mouse (93%), Rat (93%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Angpt2/Tie2 autostimulatory loop controls tumorigenesis.

EMBO molecular medicine

Karabid NM,Wiedemann T,Gulde S,Mohr H,Segaran RC,Geppert J,Rohm M,Vitale G,Gaudenzi G,Dicitore A,Ankerst DP,Chen Y,Braren R,Kaissis G,Schilling F,Schillmaier M,Eisenhofer G,Herzig S,Roncaroli F,Honegger JB,Pellegata NS

PA5-28582 was used in Western Blot, Proximity Ligation Assay to show that PitNET cells express a functional TIE2 receptor and secrete bioactive ANGPT2, which promotes, besides angiogenesis, tumor cell growth in an autocrine and paracrine fashion.

Mon May 09 00:00:00 UTC 2022

Angiopoietin1 Deficiency in Hepatocytes Affects the Growth of Colorectal Cancer Liver Metastases (CRCLM).

Cancers

Ibrahim NS,Lazaris A,Rada M,Petrillo SK,Huck L,Hussain S,Ouladan S,Gao ZH,Gregorieff A,Essalmani R,Seidah NG,Metrakos P

Published figure using TEK polyclonal antibody (Product # PA5-28582) in Immunohistochemistry

Fri Dec 20 00:00:00 UTC 2019

生物信息学

蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; hTIE2; p140 TEK; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

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基因别名: CD202B; TEK; TIE-2; TIE2; VMCM; VMCM1

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UniProt ID: (Human) Q02763

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Entrez Gene ID: (Human) 7010

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功能

protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding growth factor binding transmembrane signal receptor

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