Invitrogen

TET1 Monoclonal Antibody (GT1462)

TET1 Antibody in Immunocytochemistry (ICC/IF)

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TET1 Antibody (MA5-16312) in ICC/IF

TET1 Monoclonal Antibody (GT1462) detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (Product # MA5-16312) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TET1 Antibody in Immunohistochemistry (IHC)

TET1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

MA5-16312

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看2篇已发表文章 2篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT1462

抗原

Recombinant protein encompassing a sequence within the center region of human TET1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2537831

产品详细信息

Recommended positive controls: 293T, DDDDK-human TET1-transfected 293T, NT2D1, NT2D1 nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

MA5-16312 was used in Chromatin immunoprecipitation and Western Blotting to study how TET enzymes are recruited and regulated at genomic enhancers and CpG islands.

Sat Jan 01 00:00:00 UTC 2022

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

MA5-16312 was used in Chromatin immunoprecipitation and Western Blotting to study how TET enzymes are recruited and regulated at genomic enhancers and CpG islands.

Sat Jan 01 00:00:00 UTC 2022

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

MA5-16312 was used in Chromatin immunoprecipitation and Western Blotting to study how TET enzymes are recruited and regulated at genomic enhancers and CpG islands.

Sat Jan 01 00:00:00 UTC 2022

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

MA5-16312 was used in Chromatin immunoprecipitation and Western Blotting to study how TET enzymes are recruited and regulated at genomic enhancers and CpG islands.

Sat Jan 01 00:00:00 UTC 2022

Ten-Eleven Translocation 1 Promotes Malignant Progression of Cholangiocarcinoma With Wild-Type Isocitrate Dehydrogenase 1.

Hepatology (Baltimore, Md.)

Bai X,Zhang H,Zhou Y,Nagaoka K,Meng J,Ji C,Liu D,Dong X,Cao K,Mulla J,Cheng Z,Mueller W,Bay A,Hildebrand G,Lu S,Wallace J,Wands JR,Sun B,Huang CK

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in Immunohistochemistry

Sat May 01 00:00:00 UTC 2021

Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.

Proceedings of the National Academy of Sciences of the United States of America

Sengupta S,Wang H,Yang C,Szczesny B,Hegde ML,Mitra S

MA5-16312 was used in Immunoprecipitation to show induction of oxidized bases and single-strand breaks (SSBs), but not direct double-strand breaks (DSBs), in the genome during gene activation by ligands of the nuclear receptor superfamily.

Tue Sep 08 00:00:00 UTC 2020

Integrin α6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells.

Oncogene

Herrmann A,Lahtz C,Song J,Aftabizadeh M,Cherryholmes GA,Xin H,Adamus T,Lee H,Grunert D,Armstrong B,Chu P,Brown C,Lim M,Forman S,Yu H

MA5-16312 was used in Western Blotting to conclude that ECM- integrin α6-STAT3-TET3 axis regulates hydroxymethylation of genes important for GSCs, thereby increasing GSC tumorigenicity and resistance to therapies.

Sun Mar 01 00:00:00 UTC 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 UTC 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 UTC 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 UTC 2020

Vitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation.

Scientific reports

Kouakanou L,Peters C,Sun Q,Floess S,Bhat J,Huehn J,Kabelitz D

MA5-16312 was used in Flow Cytometry to investigate the influence of phospho-modified Vitamin C on the regulatory activity of human Vγ9 Vδ2 T cells and TGF-β-induced FOXP3 expression.

Thu Apr 16 00:00:00 UTC 2020

Hemodynamic disturbed flow induces differential DNA methylation of endothelial Kruppel-Like Factor 4 promoter in vitro and in vivo.

Circulation research

Jiang YZ,Jiménez JM,Ou K,McCormick ME,Zhang LD,Davies PF

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Fri Jun 20 00:00:00 UTC 2014

生物信息学

蛋白别名: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; Ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

查看更多收起

基因别名: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

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UniProt ID: (Human) Q8NFU7, (Mouse) Q3URK3

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Entrez Gene ID: (Human) 80312, (Mouse) 52463

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功能

iron ion binding zinc ion binding structure-specific DNA binding methylcytosine dioxygenase activity DNA binding protein binding oxidoreductase activity metal ion binding dioxygenase activity

参与通路

inner cell mass cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated protein O-linked glycosylation chromatin modification stem cell population maintenance regulation of DNA methylation positive regulation of transcription from RNA polymerase II promoter oxidation-reduction process DNA demethylation negative regulation of methylation-dependent chromatin silencing regulation of genetic imprinting positive regulation of cell proliferation covalent chromatin modification positive regulation of histone methylation

TET1 Monoclonal Antibody (GT1462)

TET1 Antibody (MA5-16312) in ICC/IF

产品信息

MA5-16312

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

TET1 Monoclonal Antibody (GT1462)

TET1 Antibody (MA5-16312) in ICC/IF

产品信息

MA5-16312

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)