Invitrogen

TFAM Polyclonal Antibody

TFAM Antibody in Immunocytochemistry (ICC/IF)

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TFAM Antibody (PA5-23776) in ICC/IF

Immunocytochemistry analysis of TFAM in NCI-H460 cells. Samples were incubated with TFAM polyclonal antibody (Product # PA5-23776) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). TFA... View More

TFAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-23776

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫组织化学（悬浮细胞） (IHC (Free))

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免疫细胞化学 (ICC/IF)

1:10-1:50

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流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.38 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2541276

靶标信息

Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Prevention of HIV-1 TAT Protein-Induced Peripheral Neuropathy and Mitochondrial Disruption by the Antimuscarinic Pirenzepine.

Frontiers in neurology

Han MM,Frizzi KE,Ellis RJ,Calcutt NA,Fields JA

Published figure using TFAM polyclonal antibody (Product # PA5-23776) in Western Blot

Tue Jul 06 00:00:00 UTC 2021

Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: Implications for therapeutic targeting of astroglia.

Neurobiology of disease

Swinton MK,Carson A,Telese F,Sanchez AB,Soontornniyomkij B,Rad L,Batki I,Quintanilla B,Pérez-Santiago J,Achim CL,Letendre S,Ellis RJ,Grant I,Murphy AN,Fields JA

PA5-23776 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to investigate alterations in mitochondrial biogenesis in neurons and astroglia in postmortem brain specimens from a cohort of HIV associated neurocognitive disorder decedents that were on antiretroviral therapy.

Tue Oct 01 00:00:00 UTC 2019

Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: Implications for therapeutic targeting of astroglia.

Neurobiology of disease

Swinton MK,Carson A,Telese F,Sanchez AB,Soontornniyomkij B,Rad L,Batki I,Quintanilla B,Pérez-Santiago J,Achim CL,Letendre S,Ellis RJ,Grant I,Murphy AN,Fields JA

Tue Oct 01 00:00:00 UTC 2019

Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: Implications for therapeutic targeting of astroglia.

Neurobiology of disease

Swinton MK,Carson A,Telese F,Sanchez AB,Soontornniyomkij B,Rad L,Batki I,Quintanilla B,Pérez-Santiago J,Achim CL,Letendre S,Ellis RJ,Grant I,Murphy AN,Fields JA

Tue Oct 01 00:00:00 UTC 2019

Tenofovir disoproxil fumarate induces peripheral neuropathy and alters inflammation and mitochondrial biogenesis in the brains of mice.

Scientific reports

Fields JA,Swinton MK,Carson A,Soontornniyomkij B,Lindsay C,Han MM,Frizzi K,Sambhwani S,Murphy A,Achim CL,Ellis RJ,Calcutt NA

PA5-23776 was used in Immunohistochemistry - Free Floating to study how Tenofovir disoproxil fumarate contributes to neurotoxicity, identifying functions in which it alters inflammation and mitochondrial biogenesis in vivo.

Wed Nov 20 00:00:00 UTC 2019

Tenofovir disoproxil fumarate induces peripheral neuropathy and alters inflammation and mitochondrial biogenesis in the brains of mice.

Scientific reports

Fields JA,Swinton MK,Carson A,Soontornniyomkij B,Lindsay C,Han MM,Frizzi K,Sambhwani S,Murphy A,Achim CL,Ellis RJ,Calcutt NA

Wed Nov 20 00:00:00 UTC 2019

SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy.

PloS one

Jevtic V,Kindle P,Avilov SV

PA5-23776 was used in Immunocytochemistry to test and demonstrate the suitability of SYBR Gold dye in labeling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy.

Thu Feb 28 00:00:00 UTC 2019

生物信息学

蛋白别名: Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial

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基因别名: MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM

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UniProt ID: (Human) Q00059

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Entrez Gene ID: (Human) 7019

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功能