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TFAP4 Antibody (PA5-116031) in ICC/IF
Immunocytochemistry analysis of TFAP4 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116031) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-116031
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TFAP4(Accession Q01664), corresponding to amino acid residues E290-P338.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900665
产品详细信息
Antibody detects endogenous levels of total TFAP4.
靶标信息
Transcription factors of the basic helix-loop-helix-zipper(bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activating enhancer-binding protein 4; bHLHc41; Class C basic helix-loop-helix protein 41; Transcription factor AP-4; transcription factor AP-4 (activating enhancer binding protein 4); transcription factor AP-4 (activating enhancer-binding protein 4); transcription factor AP4
基因别名: AI642933; AP-4; BHLHC41; D930048N17Rik; Tcfap4; TFAP4
UniProt ID: (Human) Q01664
Entrez Gene ID: (Human) 7023, (Mouse) 83383, (Rat) 360482
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
E-box binding
transcription factor activity, sequence-specific DNA binding
DNA-binding transcription factor
transcription from RNA polymerase II promoter
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
negative regulation of cell proliferation
positive regulation of apoptotic process
negative regulation of DNA binding
negative regulation by host of viral transcription
positive regulation by host of viral transcription
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of cell cycle arrest
cellular response to dexamethasone stimulus
positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
