Invitrogen

TFF3 Polyclonal Antibody

TFF3 Antibody in Immunocytochemistry (ICC/IF)

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TFF3 Antibody (PA5-21081) in ICC/IF

Immunofluorescence analysis of Trefoil factor 3 was performed using 70% confluent log phase LoVo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TFF3 Polyclonal Antibody (Product # PA5-21081) at 10 µg/mL in 0.1% BSA, incubated at 4-degree Celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product #... View More

TFF3 Antibody in Immunohistochemistry (IHC)

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TFF3 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-TFF3 Polyclonal Antibody (Product # PA5-21081), shows a higher level of TFF3 expression in the LoVo cell line as compared to HT-29. (doi: 10.3892/ijo.2019.4854) {RE}

产品信息

PA5-21081

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

2 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

2-20 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 16 amino acid peptide near the carboxy terminus of human TFF3.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152862

产品详细信息

A suggested positive control is rat small intestine tissue lysate.

PA5-21081 can be used with blocking peptide PEP-1195.

靶标信息

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-21081 was used in Immunohistochemistry and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor developmen.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

Fri Feb 03 00:00:00 UTC 2023

Maternal administration of probiotics promotes gut development in mouse offsprings.

PloS one

Yu Y,Lu J,Oliphant K,Gupta N,Claud K,Lu L

PA5-21081 was used in Immunohistochemistry to investigate if maternal administration of probiotics to pregnant mouse dams can recapitulate the beneficial effects observed in neonates fed with probiotics directly.

Thu Oct 08 00:00:00 UTC 2020

Maternal administration of probiotics promotes gut development in mouse offsprings.

PloS one

Yu Y,Lu J,Oliphant K,Gupta N,Claud K,Lu L

Thu Oct 08 00:00:00 UTC 2020

Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hwang D,Jo H,Hwang S,Kim JK,Kim IH,Lim YH

PA521081 was used in western blot to determine effect of oxyresveratrol on intestinal tight junctions through stimulation of trefoil factor 3 production in goblet cells

Sun Jan 01 00:00:00 UTC 2017

IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells.

Investigative ophthalmology & visual science

Tukler Henriksson J,Coursey TG,Corry DB,De Paiva CS,Pflugfelder SC

PA5-21081 was used in Immunohistochemistry-immunofluorescence to demonstrate that conjunctival goblet cells are IL-13 responsive cells that produce factors known to maintain epithelial barrier, stimulate mucin production, and modulate immune response in nonocular mucosa when treated with IL-13.