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THRA Antibody (PA1-216) in ICC/IF
Immunofluorescent analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thyroid Hormone Receptor alpha-2 polyclonal antibody (Product # PA1-216) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamb... View More
产品信息
PA1-216
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325034
产品详细信息
PA1-216 detects the thyroid hormone receptor alpha 2 from human and mouse samples.
PA1-216 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects bands at ~58 kDa representing Tra2 in mouse tissue. This antibody also detects a ~43 kDa protein which is believed to be non-specific.
The PA1-216 immunizing peptide corresponds to amino acid residues 455-469 from rat thyroid hormone receptor alpha 2.
靶标信息
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: c-erbA-1; c-erbA-alpha; c-erbAalpha; cerbA protein; cerbAalpha; E ERB-T-1; EAR-7; ERBA ALPHA; ERBA related 7; ERBA-related 7; MGC000261; MGC43240; Nuclear receptor subfamily 1 group A member 1; Oncogene ERBA; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; TR; TR alpha 1; TR alpha 1+2; TR alpha 2; triiodothyronine receptor; V-erbA-related protein 7
基因别名: 6430529J03Rik; AR7; AW259572; c-ERBA-1; C-erba-alpha; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; Rvr; T3R[a]; T3Ralpha; THRA; THRA1; THRA2
UniProt ID: (Human) P10827, (Mouse) P63058
Entrez Gene ID: (Human) 7067, (Mouse) 21833
transcription regulatory region sequence-specific DNA binding
steroid receptor RNA activator RNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
thyroid hormone receptor activity
protein binding
transcription factor binding
zinc ion binding
TBP-class protein binding
protein domain specific binding
chromatin DNA binding
protein complex binding
protein homodimerization activity
transcription regulatory region DNA binding
intronic transcription regulatory region DNA binding
protein heterodimerization activity
thyroid hormone binding
DNA binding
single-stranded RNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
sequence-specific DNA binding
metal ion binding
protein dimerization activity
cartilage condensation
ossification
regulation of thyroid hormone mediated signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
learning or memory
regulation of heart contraction
female courtship behavior
response to cold
hormone-mediated signaling pathway
organ morphogenesis
positive regulation of myotube differentiation
negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
erythrocyte differentiation
intracellular receptor signaling pathway
thyroid gland development
regulation of myeloid cell apoptotic process
cytoplasmic sequestering of transcription factor
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
positive regulation of female receptivity
positive regulation of transcription from RNA polymerase II promoter
regulation of lipid catabolic process
Type I pneumocyte differentiation
negative regulation of DNA-templated transcription, initiation
kidney development
liver development
transcription initiation from RNA polymerase II promoter
brain development
adrenal gland development
response to nutrient levels
response to drug
digestive tract development
embryonic organ development
