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TID1 Antibody (PA5-96554) in WB
Western blot analysis of TID1 in extracts of various cell lines. Samples were incubated with TID1 Polyclonal antibody (Product # PA5-96554) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-96554
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_0051383)
偶联物
形式
Liquid
浓度
0.4 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808356
产品详细信息
Immunogen sequence: AKGVNKEFTV NIMDTCERCN GKGNEPGTKV QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL FISIAQALLG GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG YGDHYIHIKI RVPKRLTSRQ QSLILSYAED ETDVEGTVNG VTLTSSGGST MDSSAGSKAR REAGEDEEGF LSKLKKMFTS; Positive Samples: BT-474, Jurkat, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver; Cellular Location: Cell junction, Cytoplasm, Mitochondrion matrix, Peripheral membrane protein, cytosol, postsynaptic cell membrane, synapse
靶标信息
TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; hTid-1; mTid-1; Tumorous imaginal discs protein Tid56 homolog
基因别名: 1200003J13Rik; 1810053A11Rik; C81173; DNAJA3; HCA57; hTID-1; Tid-1; TID1; Tid1l
UniProt ID: (Human) Q96EY1, (Mouse) Q99M87
Entrez Gene ID: (Human) 9093, (Mouse) 83945, (Rat) 360481
protein binding
ATP binding
Hsp70 protein binding
GTPase regulator activity
receptor tyrosine kinase binding
metal ion binding
unfolded protein binding
interferon-gamma receptor binding
transcription factor binding
protein kinase binding
heat shock protein binding
NF-kappaB binding
chaperone binding
chaperone
negative regulation of transcription from RNA polymerase II promoter
mitochondrial DNA replication
protein folding
activation-induced cell death of T cells
mitochondrion organization
small GTPase mediated signal transduction
cell aging
negative regulation of cell proliferation
response to heat
T cell differentiation in thymus
positive regulation of T cell proliferation
negative regulation of programmed cell death
regulation of catalytic activity
skeletal muscle acetylcholine-gated channel clustering
negative regulation of protein kinase activity
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
neuromuscular junction development
embryo development
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
response to interferon-gamma
protein refolding
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein stabilization
negative regulation of interferon-gamma-mediated signaling pathway
