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TIE2 Antibody (PA5-47963) in IHC (P)
Immunohistochemical analysis of TIE2 in immersion fixed whole mount zebrafish embryo (24 hours old). Samples were incubated in TIE2 polyclonal antibody (Product # PA5-47963) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
产品信息
PA5-47963
应用
建议稀释比
已发表文章
产品规格
种属反应
Zebrafish
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant zebrafish Tie-2 Val22-His741
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610114
产品详细信息
In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Tie-2, recombinant mouse Tie-2, and rhTie-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Angiopoietin-1 receptor; CD202b antigen; EC 2.7.10; EC 2.7.10.1; endothelium-specific receptor tyrosine kinase 2; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor Tie-2
基因别名: tek; tie-2; tie2
UniProt ID: (Zebrafish) O73791
Entrez Gene ID: (Zebrafish) 30747
angiogenesis
endothelial cell proliferation
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
heart development
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
phosphorylation
positive regulation of kinase activity
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of protein kinase B signaling
