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TIE2 (TEK) Antibody (PA5-80103) in WB
Western blot was performed using TEK Polyclonal Antibody (Product # PA5-80103) and a 140 kDa band corresponding to TEK/TIE2 was observed across tissues tested. Whole cell extracts (30 µg lysate) of Mouse lung (Lane 1), Mouse kidney (Lane 2), Mouse adipose(Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilut... View More
产品信息
PA5-80103
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human TIE2 recombinant protein (Position: Q641-I830).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2747218
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; hTIE2; HYK; mTIE2; p140 TEK; STK1; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2
基因别名: AA517024; CD202B; Hyk; STK1; TEK; TIE-2; TIE2; VMCM; VMCM1
UniProt ID: (Human) Q02763, (Mouse) Q02858
Entrez Gene ID: (Human) 7010, (Mouse) 21687
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
growth factor binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
MAPK cascade
angiogenesis
response to hypoxia
positive regulation of protein phosphorylation
endothelial cell proliferation
positive regulation of endothelial cell proliferation
endochondral ossification
sprouting angiogenesis
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
heart development
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
negative regulation of angiogenesis
peptidyl-tyrosine phosphorylation
organ regeneration
regulation of establishment or maintenance of cell polarity
substrate adhesion-dependent cell spreading
negative regulation of apoptotic process
regulation of vascular permeability
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
response to estrogen
positive regulation of angiogenesis
protein autophosphorylation
Tie signaling pathway
negative regulation of inflammatory response
leukocyte migration
protein oligomerization
response to cAMP
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
definitive hemopoiesis
heart trabecula formation
positive regulation of ERK1 and ERK2 cascade
positive regulation of intracellular signal transduction
positive regulation of actin cytoskeleton reorganization
regulation of endothelial cell apoptotic process
negative regulation of endothelial cell apoptotic process
patterning of blood vessels
vasculogenesis
regulation of endothelial cell proliferation
positive regulation of cytokine secretion involved in immune response
protein phosphorylation
cell-matrix adhesion
phosphorylation
single organismal cell-cell adhesion
hemopoiesis
regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
response to retinoic acid
positive regulation of peptidyl-serine phosphorylation
intracellular signal transduction
positive regulation of protein import into nucleus
regulation of angiogenesis
positive regulation of cell adhesion
regulation of NIK/NF-kappaB signaling
