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TIGIT Chimeric Antibody (MA5-48199) in WB
Western Blot analysis of TIGIT was performed using TIGIT Chimeric Monoclonal Antibody (Product # MA5-48199). RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with TIGIT Chimeric Monoclonal Antibody at 0.3 µg/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
产品信息
MA5-48199
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1B4
抗原
This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074642
产品详细信息
Specificity: This antibody is specific for murine TIGIT.
靶标信息
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T-cell immunoreceptor with Ig and ITIM domains; tigit; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3
基因别名: ENSMUSG00000071552; Vstm3
Entrez Gene ID: (Mouse) 100043314
