Invitrogen

TIMP2 Recombinant Rabbit Monoclonal Antibody (JM87-10)

TIMP2 Antibody in Immunocytochemistry (ICC/IF)

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TIMP2 Antibody (MA5-32632) in ICC/IF

Immunofluorescence analysis of TIMP2 was performed using 70% confluent log phase HT-1080 cells treated with Protein Transport Inhibitor (PTI; 1x for 4 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP2 Recombinant Rabbit Monoclonal Antibody (Product # MA5-32632) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heav... View More

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TIMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of TIMP2 was observed in SH-SY5Y and HT-1080 as compared to Daudi using Anti-TIMP2 Recombinant Rabbit Monoclonal Antibody (Product # MA5-32632) in Western Blot. {RE}

产品信息

MA5-32632

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM87-10

抗原

Synthetic peptide within Human TIMP2 aa 171-220.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809909

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。