Invitrogen

TIMP4 Polyclonal Antibody

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TIMP4 Antibody (PA5-92959) in WB

Western blot analysis of TIMP4 in extracts of various cell lines. Samples were incubated with TIMP4 Polyclonal antibody (Product # PA5-92959) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-92959

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

- -

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_0032471)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.48 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2806560

产品详细信息

Immunogen sequence: CSCAPAHPQQ HICHSALVIR AKISSEKVVP ASADPADTEK MLRYEIKQIK MFKGFEKVKD VQYIYTPFDS SLCGVKLEAN SQKQYLLTGQ VLSDGKVFIH LCNYIEPWED LSLVQRESLN HHYHLNCGCQ ITTCYTVPCT ISAPNECLWT DWLLERKLYG YQAQHYVCMK HVDGTCSWYR GHLPLRKEFV DIVQP; Positive Samples: HT-29, Mouse heart, Mouse brain, Mouse ovary, Rat heart, Rat brain

靶标信息

Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3. It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases. Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix. TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor. The netrin domain of TIMP4 is involved in platelet recruitment and aggregation. Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.

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