Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
TIP60 Antibody (PA5-34548) in ICC/IF
Immunofluorescence analysis of TIP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-TIP60 Rabbit Polyclonal Antibody (Product # PA5-34548) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
产品信息
PA5-34548
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A 15 amino acid peptide near the carboxy terminus of human KAT5
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2551900
产品详细信息
A suggested positive control is human brain tissue lysate.
PA5-34548 can be used with blocking peptide PEP-1591.
靶标信息
TIP60 is a transcription factor, originally isolated as an HIV-1 TAT-interactive protein, known to interact with the viral TAT protein that leads to HTATIP polyubiquitination and targets it for degradation. It belongs to the MYST family of histone acetyl transferases (HATs). TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. A co-regulator of transcription factors, TIP60 either promotes or suppresses tumorigenesis. TIP60 is an enzyme that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 60 kDa Tat-interactive protein; cPLA(2)-interacting protein; cPLA2 interacting protein; Histone acetyltransferase HTATIP; Histone acetyltransferase KAT5; HIV-1 Tat interactive protein; HIV-1 tat interactive protein 1, 60 kDa homolog; HIV-1 tat interactive protein 1, homolog; HIV-1 Tat interactive protein 60 kD; HIV-1 Tat interactive protein, 60 kD; HIV-1 Tat interactive protein, 60kDa; HIV-1 tat interactive protein, homolog; K(lysine) acetyltransferase 5; K-acetyltransferase 5; Lysine acetyltransferase 5; Protein 2-hydroxyisobutyryltransferase KAT5; Protein acetyltransferase KAT5; Protein crotonyltransferase KAT5; Tat interacting protein, 60kDa; Tat-interactive protein-60; Tip60; TIP60(HTATIP)
基因别名: AI839539; CPLA2; ESA1; HTATIP; HTATIP1; KAT5; PLIP; TIP; Tip55; TIP60; Tip60b; ZC2HC5
UniProt ID: (Human) Q92993, (Mouse) Q8CHK4, (Rat) Q99MK2
Entrez Gene ID: (Human) 10524, (Mouse) 81601, (Rat) 192218
transcription coactivator activity
histone acetyltransferase activity
protein binding
acetyltransferase activity
metal ion binding
androgen receptor binding
repressing transcription factor binding
chromatin binding
transcription factor binding
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
protein complex binding
phospholipase binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
DNA replication
double-strand break repair
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
response to ionizing radiation
viral process
histone acetylation
androgen receptor signaling pathway
negative regulation of interleukin-2 production
regulation of growth
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
regulation of signal transduction by p53 class mediator
positive regulation of protein acetylation
beta-catenin-TCF complex assembly
regulation of transcription, DNA-templated
chromatin modification
apoptotic process
response to heat
histone H4 acetylation
cellular response to hydrogen peroxide
cellular response to X-ray
